PUBLICATION
Comprehensive gene and microRNA expression profiling on cardiovascular system in zebrafish co-exposured of SiNPs and MeHg
- Authors
- Hu, H., Shi, Y., Zhang, Y., Wu, J., Asweto, C.O., Feng, L., Yang, X., Duan, J., Sun, Z.
- ID
- ZDB-PUB-170716-1
- Date
- 2017
- Source
- The Science of the total environment 607-608: 795-805 (Journal)
- Registered Authors
- Keywords
- Cardiovascular system, Combined toxicity, MeHg, SiNPs, Transcriptional analysis
- MeSH Terms
-
- Animals
- Cardiovascular System/drug effects*
- Gene Expression Profiling*
- Methylmercury Compounds/toxicity*
- MicroRNAs/genetics*
- Nanoparticles/toxicity
- RNA, Messenger/genetics*
- Silicon Dioxide/toxicity*
- Zebrafish
- PubMed
- 28711009 Full text @ Sci. Total Environ.
- CTD
- 28711009
Citation
Hu, H., Shi, Y., Zhang, Y., Wu, J., Asweto, C.O., Feng, L., Yang, X., Duan, J., Sun, Z. (2017) Comprehensive gene and microRNA expression profiling on cardiovascular system in zebrafish co-exposured of SiNPs and MeHg. The Science of the total environment. 607-608:795-805.
Abstract
Air pollution has been shown to increase cardiovascular diseases. However, little attention has been paid to the combined effects of PM and air pollutants on the cardiovascular system. To explore this, a high-throughput sequencing technology was used to determine combined effects of silica nanoparticles (SiNPs) and MeHg in zebrafish. Our study demonstrated that SiNPs and MeHg co-exposure could cause significant changes in mRNA and miRNA expression patterns in zebrafish. The differentially expressed (DE) genes in profiles 17 and 26 of STC analysis suggest that SiNPs and MeHg co-exposure had more proinflammatory and cardiovascular toxicity in zebrafish than single exposure. Major gene functions associated with cardiovascular system in the co-exposed zebrafish were discerned from the dynamic-gene-network, including stxbp1a, celf4, ahr1b and bai2. In addition, the prominently expressed pathway of cardiac muscle contraction was targeted by 3 DE miRNAs identified by the miRNA-pathway-network (dre-miR-7147, dre-miR-26a and dre-miR-375), which included 23 DE genes. This study presents a global view of the combined SiNPs and MeHg toxicity on the dynamic expression of both mRNAs and miRNAs in zebrafish, and could serve as fundamental research clues for future studies, especially on cardiovascular system toxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping