PUBLICATION
Anti-inflammatory properties of extracts from Chimonanthus nitens Oliv. leaf
- Authors
- Sun, Q., Zhu, J., Cao, F., Chen, F.
- ID
- ZDB-PUB-170713-2
- Date
- 2017
- Source
- PLoS One 12: e0181094 (Journal)
- Registered Authors
- Keywords
- Zebrafish, Neutrophils, Macrophages, Inflammation, Leaves, Cytokines, Larvae, Sugar acids
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/chemistry*
- Anti-Inflammatory Agents/pharmacology*
- Interleukin-1beta/genetics
- Interleukin-6/genetics
- Mice
- Neutrophil Infiltration/drug effects
- Plant Extracts/chemistry*
- Plant Extracts/pharmacology*
- Plant Leaves/chemistry*
- RAW 264.7 Cells
- RNA, Messenger/genetics
- Tandem Mass Spectrometry
- Tumor Necrosis Factor-alpha/genetics
- Zebrafish
- PubMed
- 28700722 Full text @ PLoS One
Citation
Sun, Q., Zhu, J., Cao, F., Chen, F. (2017) Anti-inflammatory properties of extracts from Chimonanthus nitens Oliv. leaf. PLoS One. 12:e0181094.
Abstract
Chimonanthus nitens Oliv. (CN) is a species in the family Calycanthaceae. Its leaf is widely used to make traditional herbal tea in southern China and has a wide range of therapeutic effects. The profile of the ethanol extracts from CN leaves was identified by UPLC-QTOF-MS/MS. Forty seven compounds were determined including organic acids, phenolic acids and derivatives, flavonoids, coumarins, fatty acids and other compounds. The effect of the CN extracts on the inflammatory damage in zebrafish and in RAW 264.7 cells was investigated. The extracts demonstrated a strong ability to inhibit the recruitment of neutrophils in LPS-stimulated zebrafish, but macrophage migration was not significantly affected. Pro-inflammatory cytokines (i.e., TNF-α, IL-6 and IL-1β) were also determined by q-PCR. The extracts strongly reduced mRNA expression of TNF-α, IL-6 but not IL-1β in zebrafish model, while significantly inhibited the production of the factors in the RAW 264.7 cells. Therefore, our results suggest that the ethanol extracts of CN leaves may serve as a source of nutraceutical compounds with anti-inflammatory properties.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping