|ZFIN ID: ZDB-PUB-170713-1|
OPTiM: Optical projection tomography integrated microscope using open-source hardware and software
Watson, T., Andrews, N., Davis, S., Bugeon, L., Dallman, M.D., McGinty, J.
|Source:||PLoS One 12: e0180309 (Journal)|
|Registered Authors:||Andrews, Natalie, Bugeon, Laurence, Dallman, Maggie|
|Keywords:||Zebrafish, Optical lenses, Fluorescence imaging, Inverted microscopy, Light microscopy, Relays, Scanning confocal microscopy, Focal planes|
|PubMed:||28700724 Full text @ PLoS One|
Watson, T., Andrews, N., Davis, S., Bugeon, L., Dallman, M.D., McGinty, J. (2017) OPTiM: Optical projection tomography integrated microscope using open-source hardware and software. PLoS One. 12:e0180309.
ABSTRACTWe describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1.6 μm). All three methods use the microscope's existing incoherent light source (i.e. arc-lamp) and all of its inherent functionality is maintained for day-to-day use. OPT acquisitions are performed on in vivo zebrafish embryos to demonstrate the implementations' viability.