PUBLICATION

Resveratrol ameliorates diet-induced dysregulation of lipid metabolism in zebrafish (Danio rerio)

Authors
Ran, G., Ying, L., Li, L., Yan, Q., Yi, W., Ying, C., Wu, H., Ye, X.
ID
ZDB-PUB-170708-6
Date
2017
Source
PLoS One   12: e0180865 (Journal)
Registered Authors
Keywords
Zebrafish, Autophagic cell death, Lipid metabolism, Body weight, Blood plasma, Blood sugar, Muscle proteins, Obesity
MeSH Terms
  • Animals
  • Antioxidants/pharmacology*
  • Caloric Restriction/adverse effects
  • Caveolin 1/genetics
  • Caveolin 1/metabolism
  • Diet/methods*
  • Eating/physiology
  • Female
  • Gene Expression Regulation/drug effects*
  • Lipid Metabolism/drug effects*
  • Lipid Metabolism/genetics
  • Male
  • Microtubule-Associated Proteins/genetics
  • Microtubule-Associated Proteins/metabolism
  • PPAR gamma/genetics
  • PPAR gamma/metabolism
  • Phosphorylation
  • Protein Kinases/genetics
  • Protein Kinases/metabolism
  • Signal Transduction
  • Sirtuin 1/genetics
  • Sirtuin 1/metabolism
  • Stilbenes/pharmacology*
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Triglycerides/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
28686680 Full text @ PLoS One
Abstract
Defective lipid metabolism is associated with increased risk of various chronic diseases, such as obesity, cardiovascular diseases, and diabetes. Resveratrol (RSV), a natural polyphenol, has been shown the potential of ameliorating disregulations of lipid metabolism. The objective of this study was to investigate the effects of feed intake and RSV on lipid metabolism in zebrafish (Danio rerio). The adult males were randomly allocated to 6 groups: control (Con, 8 mg cysts/fish/day), control with 20 μmol/L RSV (Con+RSV), calorie restriction (CR, 5 mg cysts/fish/day), calorie restriction with RSV (CR+RSV), overfeed (OF, 60 mg cysts/fish/day), and overfeed with RSV (OF+RSV) groups. The treatment period was 8 weeks. Results showed that CR reduced body length, body weight, and condition factor of zebrafish. CR reduced levels of plasma triglyceride (TG) and induced protein expression of phosphorylated AMP-activated protein kinase-α (pAMPKα), silent information regulator 2 homolog 1 (Sirt1), and peroxisome proliferator activated receptor gamma coactivator-1α (PGC1α). RSV attenuated CR-induced pAMPKα/AMPKαincreases. RSV increased levels of Sirt1 protein in the OF zebrafish, and decreased OF-induced increase in peroxisome proliferator-activated receptor-γ (PPARγ) protein level. Additionally, RSV down-regulated caveolin-1 and up-regulated microtubule-associated protein 1 light chain 3 -II (LC3-II) protein levels in OF zebrafish. In conclusion, these results suggest that 1) CR reduces plasma TG level through activation of the AMPKα-Sirt1- PGC1α pathway; 2) under different dietary stress conditions RSV might regulate AMPK phosphorylation bi-directionally; 3) RSV might regulate lipid metabolism through the AMPKα-Sirt1-PPARγ pathway in OF zebrafish.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes