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ZFIN ID: ZDB-PUB-170707-10
Microarray-based bioinformatics analysis of the combined effects of SiNPs and PbAc on cardiovascular system in zebrafish
Hu, H., Zhang, Y., Shi, Y., Feng, L., Duan, J., Sun, Z.
Date: 2017
Source: Chemosphere   184: 1298-1309 (Journal)
Registered Authors:
Keywords: Bioinformatics analysis, Cardiovascular system, Combined toxicity, Microarray analysis, PbAc, SiNPs
MeSH Terms:
  • Animals
  • Cardiovascular System/drug effects*
  • Cardiovascular System/pathology
  • Computational Biology
  • Humans
  • MicroRNAs/genetics
  • Nanoparticles/toxicity*
  • No-Observed-Adverse-Effect Level
  • Organometallic Compounds/toxicity*
  • Phenotype
  • RNA, Messenger/metabolism
  • Silicon/toxicity*
  • Toxicity Tests/methods*
  • Water Pollutants, Chemical/toxicity*
  • Zebrafish/metabolism
  • Zebrafish/physiology*
PubMed: 28679150 Full text @ Chemosphere
With rapid development of nanotechnology and growing environmental pollution, the combined toxic effects of SiNPs and pollutants of heavy metals like lead have received global attentions. The aim of this study was to explore the cardiovascular effects of the co-exposure of SiNPs and lead acetate (PbAc) in zebrafish using microarray and bioinformatics analysis. Although there was no other obvious cardiovascular malformation except bleeding phenotype, bradycardia, angiogenesis inhibition and declined cardiac output in zebrafish co-exposed of SiNPs and PbAc at NOAEL level, significant changes were observed in mRNA and microRNA (miRNA) expression patterns. STC-GO analysis indicated that the co-exposure might have more toxic effects on cardiovascular system than that exposure alone. Key differentially expressed genes were discerned out based on the Dynamic-gene-network, including stxbp1a, ndfip2, celf4 and gsk3b. Furthermore, several miRNAs obtained from the miRNA-Gene-Network might play crucial roles in cardiovascular disease, such as dre-miR-93, dre-miR-34a, dre-miR-181c, dre-miR-7145, dre-miR-730, dre-miR-129-5p, dre-miR-19d, dre-miR-218b, dre-miR-221. Besides, the analysis of miRNA-pathway-network indicated that the zebrafish were stimulated by the co-exposure of SiNPs and PbAc, which might cause the disturbance of calcium homeostasis and endoplasmic reticulum stress. As a result, cardiac muscle contraction might be deteriorated. In general, our data provide abundant fundamental research clues to the combined toxicity of environmental pollutants and further in-depth verifications are needed.