PUBLICATION
A Simple and Inexpensive Microinjection System for Zebrafish Embryos and Larvae
- Authors
- Samaee, S.M., Nikkhah, H., Varga, Z.M., Rezaei, B.
- ID
- ZDB-PUB-170706-2
- Date
- 2017
- Source
- Zebrafish 14(6): 581-585 (Journal)
- Registered Authors
- Varga, Zoltan M.
- Keywords
- embryo-larva, microinjection setup, zebrafish
- MeSH Terms
-
- Aniline Compounds/administration & dosage
- Animals
- Coloring Agents/administration & dosage
- Embryo, Nonmammalian/drug effects
- Larva/drug effects
- Microinjections/methods
- Microinjections/veterinary*
- Zebrafish/embryology*
- PubMed
- 28678656 Full text @ Zebrafish
Citation
Samaee, S.M., Nikkhah, H., Varga, Z.M., Rezaei, B. (2017) A Simple and Inexpensive Microinjection System for Zebrafish Embryos and Larvae. Zebrafish. 14(6):581-585.
Abstract
Microinjection is a widely used technique to inject defined volumes and concentrations of substances and explore their physiological function in vivo. The technique has been particularly successful with zebrafish embryos; however, the injection equipment can be relatively expensive and therefore available only to well-funded laboratories. In this study, a simple, cheap, easy-to-assemble, and easy-to-use setup with a straightforward, accurate, and efficacious calibration method is introduced. The accuracy of this calibration method was tested by comparing with the results of calibration methods that are currently used in high-cost systems. Injection success with this low-cost system was verified based on the presence of injected dyes in zebrafish embryos, the absence of any significant morphological and behavioral differences between 3,4,-dichloroaniline-treated and untreated embryos, and larval viability.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping