ZFIN ID: ZDB-PUB-170706-2
A Simple and Inexpensive Microinjection System for Zebrafish Embryos and Larvae
Samaee, S.M., Nikkhah, H., Varga, Z.M., Rezaei, B.
Date: 2017
Source: Zebrafish 14(6): 581-585 (Journal)
Registered Authors: Varga, Zoltan M.
Keywords: embryo-larva, microinjection setup, zebrafish
MeSH Terms:
  • Aniline Compounds/administration & dosage
  • Animals
  • Coloring Agents/administration & dosage
  • Embryo, Nonmammalian/drug effects
  • Larva/drug effects
  • Microinjections/methods
  • Microinjections/veterinary*
  • Zebrafish/embryology*
PubMed: 28678656 Full text @ Zebrafish
ABSTRACT
Microinjection is a widely used technique to inject defined volumes and concentrations of substances and explore their physiological function in vivo. The technique has been particularly successful with zebrafish embryos; however, the injection equipment can be relatively expensive and therefore available only to well-funded laboratories. In this study, a simple, cheap, easy-to-assemble, and easy-to-use setup with a straightforward, accurate, and efficacious calibration method is introduced. The accuracy of this calibration method was tested by comparing with the results of calibration methods that are currently used in high-cost systems. Injection success with this low-cost system was verified based on the presence of injected dyes in zebrafish embryos, the absence of any significant morphological and behavioral differences between 3,4,-dichloroaniline-treated and untreated embryos, and larval viability.
ADDITIONAL INFORMATIONNo data available