|ZFIN ID: ZDB-PUB-170706-2|
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A Simple and Inexpensive Microinjection System for Zebrafish Embryos and Larvae
Samaee, S.M., Nikkhah, H., Varga, Z.M., Rezaei, B.
|Source:||Zebrafish 14(6): 581-585 (Journal)|
|Registered Authors:||Varga, Zoltan M.|
|Keywords:||embryo-larva, microinjection setup, zebrafish|
|PubMed:||28678656 Full text @ Zebrafish|
Samaee, S.M., Nikkhah, H., Varga, Z.M., Rezaei, B. (2017) A Simple and Inexpensive Microinjection System for Zebrafish Embryos and Larvae. Zebrafish. 14(6):581-585.
ABSTRACTMicroinjection is a widely used technique to inject defined volumes and concentrations of substances and explore their physiological function in vivo. The technique has been particularly successful with zebrafish embryos; however, the injection equipment can be relatively expensive and therefore available only to well-funded laboratories. In this study, a simple, cheap, easy-to-assemble, and easy-to-use setup with a straightforward, accurate, and efficacious calibration method is introduced. The accuracy of this calibration method was tested by comparing with the results of calibration methods that are currently used in high-cost systems. Injection success with this low-cost system was verified based on the presence of injected dyes in zebrafish embryos, the absence of any significant morphological and behavioral differences between 3,4,-dichloroaniline-treated and untreated embryos, and larval viability.
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