PUBLICATION
Zebrafish methanol exposure causes patterning defects and suppressive cell proliferation in retina
- Authors
- Fu, J., Jiao, J., Weng, K., Yu, D., Li, R.
- ID
- ZDB-PUB-170704-8
- Date
- 2017
- Source
- American journal of translational research 9: 2975-2983 (Journal)
- Registered Authors
- Keywords
- Zebrafish, cell proliferation, methanol, retina, retinal differentiation, small eye
- MeSH Terms
- none
- PubMed
- 28670385
Citation
Fu, J., Jiao, J., Weng, K., Yu, D., Li, R. (2017) Zebrafish methanol exposure causes patterning defects and suppressive cell proliferation in retina. American journal of translational research. 9:2975-2983.
Abstract
Purpose Methanol exposure have been shown to produce retinal abnormalities and visual dysfunctions in rodents and other mammals developing in utero. In this study, we characterized how methanol affects the retinal development in an ex utero embryonic system, the zebrafish.
Methods Zebrafish embryos were raised for 24 hours in fish water supplemented with various concentrations of methanol at 6 hours after fertilisation. The effects of methanol on retinal morphology were assessed by histologic and immunohistochemical analyses.
Results Zebrafish embryos exposed to moderate (3%) and high (4%) levels of methanol during early embryonic development had a small eye phenotype. Embryos exposed to high (4%) level of methanol had morphological abnormalities of the retinal pigment epithelia and the photoreceptors. Methanol exposure also caused inhibition of cell differentiation and proliferation in the retina at the early developmental stage.
Conclusions Low concentrations of methanol affect photoreceptor function but do not disturb retinal morphology. Higher levels of methanol exposure cause retinal patterning defects and a small eye phenotype.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping