|ZFIN ID: ZDB-PUB-170624-11|
Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos
Wu, Y., Lou, Q.Y., Ge, F., Xiong, Q.
|Source:||Scientific Reports 7: 4022 (Journal)|
|PubMed:||28642470 Full text @ Sci. Rep.|
Wu, Y., Lou, Q.Y., Ge, F., Xiong, Q. (2017) Quantitative Proteomics Analysis Reveals Novel Targets of miR-21 in Zebrafish Embryos. Scientific Reports. 7:4022.
ABSTRACTMicroRNAs (miRNAs) are noncoding RNAs which control gene expression by the suppression of translation or the degradation of mRNAs. Dre-miR-21 (miR-21) has been reported to impact cardiac valvulogenesis in zebrafish embryos. However, the target genes of miR-21 are still largely unknown. Here a tandem isobaric mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-21-regulated proteins. A total of 251 proteins were dysregulated after miR-21 knockdown, suggesting that they may be regulated by miR-21. Bioinformatics analysis indicated that these differentially expressed proteins (DEPs) participate in various biological processes, suggesting that miR-21 may be involved in diverse cellular pathways. Sixteen DEPs were also predicted to be miR-21 targets by at least two algorithms, and several candidate target genes were selected for further luciferase reporter analysis. The results showed that genes encoding tropomyosin 1 (tpm1) and poly(rC) binding protein 2 (pcbp2) are direct miR-21 targets. Taken together, our results not only reveal a large number of novel miR-21 regulated proteins that possess pleiotropic functions, but also provide novel insights into the molecular mechanisms of miR-21 regulation of zebrafish cardiac valvulogenesis and embryonic development.