PUBLICATION
Quantitative proteomic reveals the dynamic of protein profile during zebrafish final oocyte maturation
- Authors
- Ge, C., Lu, W., Chen, A.
- ID
- ZDB-PUB-170622-4
- Date
- 2017
- Source
- Biochemical and Biophysical Research Communications 490(3): 657-663 (Journal)
- Registered Authors
- Keywords
- Oocyte maturation, Proteomics, Zebrafish, iTRAQ
- MeSH Terms
-
- Animals
- Female
- Gene Expression Regulation, Developmental
- Oocytes/cytology*
- Oocytes/metabolism
- Oogenesis*
- Proteomics
- Transcriptome
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/analysis
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 28634081 Full text @ Biochem. Biophys. Res. Commun.
Citation
Ge, C., Lu, W., Chen, A. (2017) Quantitative proteomic reveals the dynamic of protein profile during zebrafish final oocyte maturation. Biochemical and Biophysical Research Communications. 490(3):657-663.
Abstract
The molecular mechanisms underlying final oocyte maturation zebrafish (Danio rerio) remain poorly understood. The present study aimed to employ iTRAQ approach for a comprehensive characterization of during zebrafish oocyte maturation proteome and for comparison between fully-grow immature and mature oocytes prior to ovulation. A total of 1568 proteins were identified, which was representing the largest zebrafish isolated oocytes proteome dataset to date. Differential expression analysis revealed 190 proteins significantly changes between immature and mature oocytes, which 136 proteins were up-regulated and 54 proteins were down-regulated in mature oocytes comparison with immature oocytes. Functional analysis revealed that these differential proteins were mostly involved in cellular response to estrogen stimulus, cellular components, extracellular region, and enzyme regulator activity, etc. The revealed differentially changes in protein expression patterns associated with oocyte maturation suggest that several of the examined proteins, such as Vitellogenin3(Vtg3), Protein S100(S100A10), 17-beta hydroxysteroid dehydrogenase(HSD17B1), Pentaxin, zona pellucida (ZP3.2), elongation factor1-alpha, Caluemnin B, and 14-3-3 protein may play a specific role during zebrafish final oocyte maturation. These data will provide powerful information for understanding the molecular mechanism underlying zebrafish oocyte maturation, and these proteins may potentially act as markers to predict control oocyte maturation of zebrafish oocytes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping