PUBLICATION
Adaptive optics improves multiphoton super-resolution imaging
- Authors
- Zheng, W., Wu, Y., Winter, P., Fischer, R., Nogare, D.D., Hong, A., McCormick, C., Christensen, R., Dempsey, W.P., Arnold, D.B., Zimmerberg, J., Chitnis, A., Sellers, J., Waterman, C., Shroff, H.
- ID
- ZDB-PUB-170621-2
- Date
- 2017
- Source
- Nature Methods 14(9): 869-872 (Journal)
- Registered Authors
- Chitnis, Ajay, Dempsey, William
- Keywords
- Optical imaging, Super-resolution microscopy
- MeSH Terms
-
- Microscopy, Fluorescence, Multiphoton/instrumentation*
- Microscopy, Fluorescence, Multiphoton/methods*
- Equipment Failure Analysis
- Image Enhancement/instrumentation*
- Image Enhancement/methods*
- Reproducibility of Results
- Feedback
- Equipment Design
- Sensitivity and Specificity
- Lenses*
- PubMed
- 28628128 Full text @ Nat. Methods
Citation
Zheng, W., Wu, Y., Winter, P., Fischer, R., Nogare, D.D., Hong, A., McCormick, C., Christensen, R., Dempsey, W.P., Arnold, D.B., Zimmerberg, J., Chitnis, A., Sellers, J., Waterman, C., Shroff, H. (2017) Adaptive optics improves multiphoton super-resolution imaging. Nature Methods. 14(9):869-872.
Abstract
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ?250 ?m from the coverslip surface.
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