PUBLICATION
Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model
- Authors
- Tandberg, J., Oliver, C., Lagos, L., Gaarder, M., Yáñez, A.J., Ropstad, E., Winther-Larsen, H.C.
- ID
- ZDB-PUB-170611-6
- Date
- 2017
- Source
- Fish & shellfish immunology 67: 189-198 (Journal)
- Registered Authors
- Keywords
- Immune response, MVs, Piscirickettsia salmonis, SRS, Vaccine, Zebrafish
- MeSH Terms
-
- Animals
- Bacterial Load
- Bacterial Vaccines/immunology*
- Cytoplasmic Vesicles/immunology
- Cytoplasmic Vesicles/metabolism*
- Female
- Fish Diseases/immunology
- Fish Diseases/prevention & control*
- Fish Proteins/genetics
- Gene Expression
- Immunity, Innate
- Male
- Models, Animal
- Piscirickettsia/immunology*
- Piscirickettsia/metabolism
- Piscirickettsiaceae Infections/immunology
- Piscirickettsiaceae Infections/prevention & control
- Piscirickettsiaceae Infections/veterinary*
- RNA, Messenger/genetics
- Sepsis/immunology
- Sepsis/prevention & control
- Sepsis/veterinary*
- Zebrafish*
- PubMed
- 28600194 Full text @ Fish Shellfish Immunol.
Citation
Tandberg, J., Oliver, C., Lagos, L., Gaarder, M., Yáñez, A.J., Ropstad, E., Winther-Larsen, H.C. (2017) Membrane vesicles from Piscirickettsia salmonis induce protective immunity and reduce development of salmonid rickettsial septicemia in an adult zebrafish model. Fish & shellfish immunology. 67:189-198.
Abstract
Infections caused by the facultative intracellular bacterial pathogen Piscirickettsia salmonis remains an unsolved problem for the aquaculture as no efficient treatments have been developed. As a result, substantial amounts of antibiotic have been used to limit salmonid rickettsial septicemia (SRS) disease outbreaks. The antibiotic usage has not reduced the occurrence, but lead to an increase in resistant strains, underlining the need for new treatment strategies. P. salmonis produce membrane vesicles (MVs); small spherical structures know to contain a variety of bacterial components, including proteins, lipopolysaccharides (LPS), DNA and RNA. MVs mimics' in many aspects their mother cell, and has been reported as alternative vaccine candidates. Here, MVs from P. salmonis was isolated and evaluated as a vaccine candidate against SRS in an adult zebrafish infection model. When zebrafish was immunized with MVs they were protected from subsequent challenge with a lethal dose of P. salmonis. Histological analysis showed a reduced bacterial load upon challenge in the MV immunized group, and the mRNA expression levels of several immune related genes altered, including mpeg1.1, tnfα, il1b, il10 and il6. The MVs induced the secretion of IgM upon immunization, indicating an immunogenic effect of the vesicles. Taken together, the data demonstrate a vaccine potential of MVs against P. salmonis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping