ZFIN ID: ZDB-PUB-170608-4
Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish
Dai, X., Shu, Y., Lou, Q., Tian, Q., Zhai, G., Song, J., Lu, S., Yu, H., He, J., Yin, Z.
Date: 2017
Source: International Journal of Molecular Sciences   18(6): (Journal)
Registered Authors: He, Jiangyan, Yin, Zhan, Zhai, Gang
Keywords: differentiation, germ cell, knockout, maintenance, tdrd12, zebrafish
MeSH Terms:
  • Animals
  • Cell Differentiation/genetics*
  • Cloning, Molecular
  • Gene Knockout Techniques
  • Gene Targeting
  • Genotype
  • Germ Cells/cytology*
  • Germ Cells/metabolism*
  • Gonads/metabolism
  • Gonads/pathology
  • Infertility/genetics
  • Meiosis
  • Mutation
  • Phylogeny
  • RNA-Binding Proteins/genetics*
  • RNA-Binding Proteins/metabolism
  • Zebrafish/classification
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed: 28590408 Full text @ Int. J. Mol. Sci.
The regularity of Piwi-interacting RNA (piRNA) biogenesis is crucial to germline development. Functioning as Piwi-interacting proteins, Tudor domain-related proteins (Tdrds) have been demonstrated to be involved in spermatogenesis and the piRNA pathway. In this study, zebrafish tdrd12 was identified, and the maternal and germ cell-specific expression patterns of zebrafish tdrd12 were observed. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent tdrd12 mutant zebrafish lines were generated. Although no defects were found during the generation of the primordial germ cells (PGCs) in the tdrd12-null fish progenies obtained from the heterozygous tdrd12 mutant parents, all Tdrd12-deficient fish developed into infertile males. The reduced numbers and eventually loss of the germ cells by 35 days post fertilization (dpf) led to masculinization and infertility of the Tdrd12-deficient fish. Meiosis defects of the germ cells in the tdrd12 mutants during the gonad-transitioning period were observed, revealing the indispensable functions of Tdrd12 in gametogenesis. Our studies demonstrated that zebrafish Tdrd12 is essential for germ cell development and maintenance.