ZFIN ID: ZDB-PUB-170603-3
Targeted disruption of aromatase reveals dual functions of cyp19a1a during sex differentiation in zebrafish
Yin, Y., Tang, H., Liu, Y., Chen, Y., Li, G., Liu, X., Lin, H.
Date: 2017
Source: Endocrinology   158(9): 3030-3041 (Journal)
Registered Authors: Chen, Yu, Liu, Xiaochun
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Aromatase/genetics
  • Aromatase/physiology*
  • Embryo, Nonmammalian
  • Estradiol/pharmacology
  • Female
  • Fertility/drug effects
  • Fertility/genetics
  • Gene Expression Regulation, Developmental/drug effects
  • Gene Transfer Techniques
  • Gonads/drug effects
  • Gonads/embryology
  • Gonads/metabolism
  • Male
  • Mutation
  • Sex Differentiation/drug effects
  • Sex Differentiation/genetics*
  • Zebrafish/embryology
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 28575219 Full text @ Endocrinology
Aromatase (encoded by the cyp19a1a and cyp19a1b genes) plays a central role in sex differentiation in fish, but its precise roles during sex differentiation are still largely unknown. Here, we systematically generated cyp19a1a and cyp19a1b mutant lines as well as a cyp19a1a;cyp19a1b double mutant line in zebrafish using transcription activator-like effectors nucleases (TALENs). Our results showed that cyp19a1a mutants and cyp19a1a;cyp19a1b double mutants, but not cyp19a1b mutants, have impaired sex differentiation, and all cyp19a1a mutants and cyp19a1a;cyp19a1b double mutants are males. During sex differentiation, the ovary-like gonads were not observed and the male sex differentiation program was delayed in the cyp19a1a-null fish, and these phenotypes could be partially rescued by 17β-estradiol treatment. Gene expression analysis indicated that male and female sex differentiation-related genes were significantly decreased in the cyp19a1a mutant. Collectively, our results revealed dual functions of the cyp19a1a gene during sex differentiation: cyp19a1a is not only indispensable for female sex differentiation but also required for male sex differentiation.