PUBLICATION
Stress-Induced Transcriptional Changes and DNA Damage Associated with Bis(2-ethylhexyl) Adipate Exposure in Zebrafish (Danio rerio) Larvae
- Authors
- Boran, H., Terzi, S.
- ID
- ZDB-PUB-170526-20
- Date
- 2017
- Source
- Bulletin of environmental contamination and toxicology 99(3): 308-314 (Journal)
- Registered Authors
- Keywords
- Acute toxicity, Bis(2-ethylhexyl) adipate, Gene expression, Genotoxicity, Zebrafish
- MeSH Terms
-
- Adipates/toxicity*
- Animals
- DNA Damage/drug effects*
- Gene Expression Regulation, Developmental/drug effects*
- Larva/drug effects
- Plasticizers
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism
- PubMed
- 28536799 Full text @ Bull. Environ. Contam. Toxicol.
Citation
Boran, H., Terzi, S. (2017) Stress-Induced Transcriptional Changes and DNA Damage Associated with Bis(2-ethylhexyl) Adipate Exposure in Zebrafish (Danio rerio) Larvae. Bulletin of environmental contamination and toxicology. 99(3):308-314.
Abstract
The present study evaluates potential toxic effects of bis(2-ethylhexyl) adipate (DEHA) plasticizer to larval (72 h post fertilization) zebrafish (Danio rerio) by analyzing changes in expression levels of stress-related genes (p53, rad51 and xrcc5) and assessing possible DNA damage of DEHA in larvae. The lethal concentration for 50% mortality (LC50) in larval zebrafish exposed for 96 h to 0-200 mg L-1 DEHA was 89.9 ± 8.03 mg L-1. A concentration-dependent increase in DNA strand breaks was detected in cells from larvae exposed for 96 h to DEHA. There were some significant differences in induction of stress-related genes in larvae exposed to DEHA relative to control.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping