PUBLICATION
In vitro characterization of grass carp (Ctenopharyngodon idella) IL-26 in regulating inflammatory factors
- Authors
- Qiu, X., Lv, M., Jian, X., Chen, D., Zhou, H., Zhang, A., Wang, X.
- ID
- ZDB-PUB-170513-4
- Date
- 2017
- Source
- Fish & shellfish immunology 66: 148-155 (Journal)
- Registered Authors
- Keywords
- Functional identification, Gene locus, Grass carp, IL-26, Pro-inflammatory property
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Carps/genetics*
- Carps/immunology*
- Carps/metabolism
- Cloning, Molecular
- DNA, Complementary/genetics
- DNA, Complementary/metabolism
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Interleukins/chemistry
- Interleukins/genetics
- Interleukins/metabolism*
- Phylogeny
- Pichia
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Recombinant Proteins/chemistry
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Sequence Alignment/veterinary
- Synteny
- PubMed
- 28495510 Full text @ Fish Shellfish Immunol.
Citation
Qiu, X., Lv, M., Jian, X., Chen, D., Zhou, H., Zhang, A., Wang, X. (2017) In vitro characterization of grass carp (Ctenopharyngodon idella) IL-26 in regulating inflammatory factors. Fish & shellfish immunology. 66:148-155.
Abstract
Interleukin 26 (IL-26) gene has been identified in human, amphibian and teleost but not in rodents. It is well accepted that IL-26 was a crucial member of IL-10 family which acts as a pro-inflammatory cytokine in human. However, the role of IL-26 in regulating inflammation in lower vertebrates including teleost has not been defined yet. In the present study, grass carp IL-26 (gcIL-26) coding sequence was isolated and identified. Its chromosomal synteny was also analyzed, showing that gcIL-26 gene is flanked by IL-22 and IFN-γ genes with the same transcriptional orientation as seen in human, amphibian and zebrafish. Given that zebrafish and grass carp IL-26 shared relatively low amino acid identities with human IL-26, the functional roles of fish IL-26 are indispensable to be elucidated. Accordingly, recombinant gcIL-26 (rgcIL-26) was prepared by using Pichia pastoris expression system, and it was found to be partially glycosylated. Using grass carp head kidney leucocytes as cell model, rgcIL-26 displayed the bioactivity to stimulate the mRNA expression of some pro-inflammatory cytokines including IL-8, IL-1β and IL-6, while inhibit mRNA expression of an anti-inflammatory cytokine, IL-10. Moreover, rgcIL-26 also up-regulated inos expression and NO production in grass carp monocytes/macrophages, strengthening its pro-inflammatory properties in fish. Those results collectively demonstrated the functional role of IL-26 in regulating inflammatory response in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping