PUBLICATION

Identification of target genes downstream of Semaphorin6A/PlexinA2 signaling in zebrafish

Authors
Emerson, S.E., St Clair, R.M., Waldron, A.L., Bruno, S.R., Duong, A., Driscoll, H.E., Ballif, B.A., McFarlane, S., Ebert, A.M.
ID
ZDB-PUB-170426-4
Date
2017
Source
Developmental dynamics : an official publication of the American Association of Anatomists   246(7): 539-549 (Journal)
Registered Authors
Keywords
Retina, eye development, microarray, proliferation, rasl11b, rx3:GFP
Datasets
GEO:GSE86246
MeSH Terms
  • Animals
  • Cell Movement
  • Cell Proliferation
  • Eye/embryology
  • Eye/growth & development
  • Gene Expression Regulation, Developmental*/genetics
  • Receptors, Cell Surface/metabolism*
  • Retina/cytology
  • Semaphorins/metabolism*
  • Signal Transduction/physiology*
  • Stem Cells
  • Zebrafish
  • Zebrafish Proteins/metabolism*
PubMed
28440030 Full text @ Dev. Dyn.
Abstract

Semaphorin (Sema)/Plexin (Plxn) signaling is important for many aspects of neuronal development, however the transcriptional regulation imposed by this signaling pathway is unknown. Previously, we identified an essential role for Sema6A/PlxnA2 signaling in regulating proliferation and cohesion of retinal precursor cells (RPCs) during early eye development. This study used RNA isolated from control, Sema6A-deficient, and PlxnA2-deficient zebrafish embryos in a microarray analysis to identify genes that were differentially expressed when this signaling pathway was disrupted.

We uncovered a set of 58 transcripts, and all but one were up-regulated in both sema6A and plxnA2 morphants. We validated gene expression changes in subset of candidates that are suggested to be involved in proliferation, migration or neuronal positioning. We further functionally evaluated one gene, rasl11b, as contributing to disrupted proliferation in sema6A and plxna2 morphants. Our results suggest rasl11b negatively regulates proliferation of RPCs in the developing zebrafish eye.
Microarray analysis has generated a resource of target genes downstream of Sema6A/PlxnA2 signaling, which can be further investigated to elucidate the downstream effects of this well-studied neuronal and vascular guidance signaling pathway.

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Errata and Notes