PUBLICATION
EpCAM controls morphogenetic programs during zebrafish pronephros development
- Authors
- Kuechlin, S., Schoels, M., Slanchev, K., Lassmann, S., Walz, G., Yakulov, T.A.
- ID
- ZDB-PUB-170416-3
- Date
- 2017
- Source
- Biochemical and Biophysical Research Communications 487(2): 209-215 (Journal)
- Registered Authors
- Slanchev, Krasimir
- Keywords
- Distal tubule, Epcam, Laser-capture microdissection, Pronephros development, RNA-seq, Zebrafish
- Datasets
- GEO:GSE97573
- MeSH Terms
-
- Zebrafish Proteins/metabolism*
- Membrane Glycoproteins/metabolism*
- Morphogenesis/physiology*
- Animals
- Gene Expression Regulation, Developmental/physiology
- Zebrafish/embryology*
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Pronephros/embryology*
- Pronephros/growth & development
- Pronephros/metabolism*
- PubMed
- 28411024 Full text @ Biochem. Biophys. Res. Commun.
Citation
Kuechlin, S., Schoels, M., Slanchev, K., Lassmann, S., Walz, G., Yakulov, T.A. (2017) EpCAM controls morphogenetic programs during zebrafish pronephros development. Biochemical and Biophysical Research Communications. 487(2):209-215.
Abstract
Epithelial cell adhesion molecule EpCAM is a transmembrane glycoprotein that is dynamically expressed in human and murine renal epithelia during development. The levels of EpCAM in the renal epithelium are upregulated both during regeneration after ischemia/reperfusion injury and in renal-derived carcinomas. The role of EpCAM in early kidney development, however, has remained unclear. The zebrafish pronephros shows a similar segmentation pattern to the mammalian metanephric nephron, and has recently emerged as a tractable model to study the regulatory programs governing early nephrogenesis. Since EpCAM shows persistent expression in the pronephros throughout early development, we developed a method to study the global changes in gene expression in specific pronephric segments of wild type and EpCAM-deficient zebrafish embryos. In epcam mutants, we found 379 differentially expressed genes. Gene ontology analysis revealed that EpCAM controls various developmental programs, including uretric bud development, morphogenesis of branching epithelium, regulation of cell differentiation and cilium morphogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping