PUBLICATION
Multi-organ toxicity induced by fine particulate matter PM2.5 in zebrafish (Danio rerio) model.
- Authors
- Duan, J., Hu, H., Zhang, Y., Feng, L., Shi, Y., Miller, M.R., Sun, Z.
- ID
- ZDB-PUB-170410-5
- Date
- 2017
- Source
- Chemosphere 180: 24-32 (Journal)
- Registered Authors
- Keywords
- Air pollution, Cardiovascular toxicity, Hepatotoxicity, Neurotoxicity, PM(2.5), Zebrafish
- MeSH Terms
-
- Air Pollutants/toxicity*
- Air Pollution/adverse effects
- Animals
- Cardiovascular System
- Cytochrome P-450 CYP1B1
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian
- Particulate Matter/metabolism
- Particulate Matter/toxicity*
- Zebrafish/embryology
- Zebrafish/metabolism
- PubMed
- 28391149 Full text @ Chemosphere
- CTD
- 28391149
Citation
Duan, J., Hu, H., Zhang, Y., Feng, L., Shi, Y., Miller, M.R., Sun, Z. (2017) Multi-organ toxicity induced by fine particulate matter PM2.5 in zebrafish (Danio rerio) model.. Chemosphere. 180:24-32.
Abstract
The fine particulate matter (PM2.5) in air pollution is a major public health concern and now known to contribute to severe diseases, therefore, a comprehensive understanding of PM2.5-induced adverse effects in living organisms is needed urgently. This study was aimed to evaluate the toxicity of PM2.5 on multi-organ systems in a zebrafish (Danio rerio) model. The embryonic toxicity induced by PM2.5 was demonstrated by an increase in mortality and inhibition of hatching rate, in a dose- and time-dependent manner. PM2.5 caused the pericardial edema, as well as reducing heart rate and cardiac output. The area of sub-intestinal vessels (SIVs) was significant reduced in Tg(fli-1:EGFP) transgenic zebrafish lines. Morphological defects and yolk sac retention were associated with hepatocyte injury. In addition, PM2.5 disrupted the axonal integrity, altering of axon length and pattern in Tg(NBT:EGFP) transgenic lines. Genes involved in cardiac function (spaw, supt6h, cmlc1), angiogenesis (vegfr2a, vegfr2b), and neural function (gabrd, chrna3, npy8br) were markedly down-regulated; while genes linked to hepatic metabolism (cyp1a, cyp1b1, cyp1c1) were significantly up-regulated by PM2.5. In summary, our data showed that PM2.5 induced the cardiovascular toxicity, hepatotoxicity and neurotoxicity in zebrafish, suggested that PM2.5 could cause multi-organ toxicity in aquatic organism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping