PUBLICATION
Oxidative stress and apotosis to zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonate (PFOS) and ZnO nanoparticles
- Authors
- Du, J., Cai, J., Wang, S., You, H.
- ID
- ZDB-PUB-170404-12
- Date
- 2017
- Source
- International journal of occupational medicine and environmental health 30: 213-229 (Journal)
- Registered Authors
- Keywords
- PFOS, ZnO nanoparticles, apoptosis, oxidative stress, perfluorooctane sulfonate, zebrafish
- MeSH Terms
-
- Alkanesulfonic Acids/toxicity*
- Animals
- Apoptosis/drug effects
- Apoptosis/genetics
- Embryo, Nonmammalian/drug effects
- Fluorocarbons/toxicity*
- Malondialdehyde
- Metal Nanoparticles/toxicity*
- Oxidative Stress/drug effects*
- Oxidative Stress/genetics
- Reactive Oxygen Species/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zinc Oxide/toxicity*
- PubMed
- 28366952 Full text @ Int J Occup Med Environ Health
Citation
Du, J., Cai, J., Wang, S., You, H. (2017) Oxidative stress and apotosis to zebrafish (Danio rerio) embryos exposed to perfluorooctane sulfonate (PFOS) and ZnO nanoparticles. International journal of occupational medicine and environmental health. 30:213-229.
Abstract
Objectives Perfluorooctane sulfonate (PFOS) and zinc oxide nanoparticles (ZnO-NPs) are frequently detected in the environment but few studies have assessed the joint toxicity of them. Oxidative stress and apoptosis to zebrafish (Danio rerio) embryos induced by the PFOS and ZnO-NPs were investigated in this study.
Material and methods The embryos were exposed to the PFOS (0, 0.4, 0.8 and 1.6 mg/l), ZnO-NPs (12.5, 25, 50 mg/l) and PFOS plus ZnO-NPs (0.4+12.5, 0.8+25 and 1.6+50 mg/l) mixture solutions until 96 h post-fertilization. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx) and malondialdehyde (MDA) content were measured in zebrafish embryos after exposure lasting for 96 h. At the same time, the genes expression related to reactive oxygen species (ROS) generation, oxidative damage and apoptosis were also measured.
Results A significant induction of the ROS accompanied by the increase in the activity of the Gpx and MDA contents were found in co-treatment groups. Furthermore, several apoptosis pathway related genes such as Bax, p53, caspase-3 and caspase-9 were significantly up-regulated in the PFOS plus ZnO-NPs exposure groups, while anti-apoptotic gene Bcl-2 was significantly down-regulated in the PFOS plus ZnO-NPs exposure groups. In addition, some oxidative stress-related genes such as Cat, GSH peroxidase 1 (Gpx1a) and superoxide dismutase 1 (Sod1) were also significantly down-regulated after the PFOS plus ZnO-NPs co-treatments.
Conclusions The results demonstrated that the PFOS plus ZnO-NPs co-exposure could cause more serious oxidative stress and apoptosis than the PFOS and ZnO-NPs exposure alone at the exposure concentrations above. The synergistic effects of the PFOS and ZnO-NPs may be the important mechanisms of their toxicity to zebrafish embryos. Int J Occup Med Environ Health 2017;30(2):213-229.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping