PUBLICATION
Functional in vivo imaging using fluorescence lifetime light-sheet microscopy
- Authors
- Mitchell, C.A., Poland, S.P., Seyforth, J., Nedbal, J., Gelot, T., Huq, T., Holst, G., Knight, R.D., Ameer-Beg, S.M.
- ID
- ZDB-PUB-170401-4
- Date
- 2017
- Source
- Optics letters 42: 1269-1272 (Journal)
- Registered Authors
- Knight, Robert
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Microscopy, Fluorescence/methods*
- Time Factors
- Zebrafish/genetics
- PubMed
- 28362747 Full text @ Opt. Lett.
Citation
Mitchell, C.A., Poland, S.P., Seyforth, J., Nedbal, J., Gelot, T., Huq, T., Holst, G., Knight, R.D., Ameer-Beg, S.M. (2017) Functional in vivo imaging using fluorescence lifetime light-sheet microscopy. Optics letters. 42:1269-1272.
Abstract
Light-sheet microscopy has become an indispensable tool for fast, low phototoxicity volumetric imaging of biological samples, predominantly providing structural or analyte concentration data in its standard format. Fluorescence lifetime imaging microscopy (FLIM) provides functional contrast, but often at limited acquisition speeds and with complex implementation. Therefore, we incorporate a dedicated frequency domain CMOS FLIM camera and intensity-modulated laser into a light-sheet setup to add fluorescence lifetime imaging functionality, allowing the rapid acquisition of volumetric data with concentration independent contrast. We then apply the system to image live transgenic zebrafish, demonstrating the capacity to rapidly collect volumetric FLIM data from an in vivo sample.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping