PUBLICATION

ankdd1b expression pattern in early stages of zebrafish development

Authors
Vaz, R., Lindstrand, A.
ID
ZDB-PUB-170327-1
Date
2017
Source
ZFIN Direct Data Submission : (Journal)
Registered Authors
Vaz, Raquel
Keywords
none
MeSH Terms
none
PubMed
none
Citation
Vaz, R., Lindstrand, A. (2017) ankdd1b expression pattern in early stages of zebrafish development. ZFIN Direct Data Submission. . (http://zfin.org).
Abstract
We performed gene expression analysis of ankdd1b by whole mount in situ hybridization during early zebrafish development. Analysis of gene expression in the developing zebrafish embryo reveals a complex expression profile that changes with time. At earlier stages, between 1 and 4 days post fertilization, ankdd1b is predominantly expressed in the skeletal muscle. At 3 days post fertilization, ankdd1b is also expressed in the pharyngeal arches and cartilaginous structures in the head. By day 4 post fertilization, expression is detected in the gill filaments and the posterior intestine. At later developmental stages ankdd1b is found predominantly in the intestine.

Method
Embryos from TL fish were collected, maintained at 28.5°C until the appropriate stage and then fixed by incubation in 4% paraformaldehyde for 2 to 3 hours at room temperature or overnight at 4°C. For embryos older than 24 hpf, pigment was removed by incubation with a solution of 3% H2O2 and 4% KOH for 10 minutes. Embryos were then dehydrated in an increasing dilutions series of methanol and stored at -20°C in 100% methanol until used. Probes for ankdd1b were obtained using the following primers: ankdd1b_fwd: 5’ CTGAAGCTTCTCGTGCAAGGTG 3’; ankdd1b_rev: 5’ GCTCCGCTATTGATGAGGAGCTG 3’. Whole mount in situ hybridization was performed, embryos were mounted in gelvatol and pictures were taken using a Zeiss Axioscope2 microscope couple with an AxioCam (Zeiss).

Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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