PUBLICATION
Anti-oxidative Responses on Hepatic Tissue of Zebrafish (Danio rerio) in a Short Duration of Sub-lethal Concentrations of Cadmium Exposure
- Authors
- Wu, S.M., Shu, L.H., Liu, J.H., Chen, C.H.
- ID
- ZDB-PUB-170321-15
- Date
- 2017
- Source
- Bulletin of environmental contamination and toxicology 98(5): 612-618 (Journal)
- Registered Authors
- Keywords
- Apoptosis, Cadmium, Metallothionein, Oxidative stress, Zebrafish
- MeSH Terms
-
- Animals
- Antioxidants/metabolism*
- Apoptosis/drug effects
- Cadmium/toxicity*
- Lipid Peroxidation/drug effects
- Liver/metabolism*
- Oxidative Stress/drug effects
- Reactive Oxygen Species/metabolism
- Zebrafish/metabolism*
- PubMed
- 28315003 Full text @ Bull. Environ. Contam. Toxicol.
Citation
Wu, S.M., Shu, L.H., Liu, J.H., Chen, C.H. (2017) Anti-oxidative Responses on Hepatic Tissue of Zebrafish (Danio rerio) in a Short Duration of Sub-lethal Concentrations of Cadmium Exposure. Bulletin of environmental contamination and toxicology. 98(5):612-618.
Abstract
The aim of the present study was to identify whether the responses of oxidative stress in zebrafish liver are similar to those in mammalians upon low doses of Cd2+ exposure in short durations. Fish were exposed to 1.78 μM Cd2+ (treatment) and 0.0 μM Cd2+ (control) for 0, 1, 3, and 6 h. The reactive oxygen species (ROS) and lipid peroxidation (LPO) of hepatic tissues significantly increased after 3 and 6 h of Cd2+ exposure, respectively. Antioxidants glutathione peroxidase (gpx1a), superoxide dismutase (sod), and catalase (cat) were up regulated after 1-3 h, and metallothionein isoforms (smtB and mt2) increased after 3-6 h of Cd2+ exposure. The caspase-3 and p53 mRNA expressions significantly increased threefolds after 1 h of Cd2+ exposure. Results confirmed that oxidative stress in the hepatic tissue was induced by Cd2+ within 3 h. However, anti-oxidative functions immediately up regulated, causing cell apoptosis levels to decrease after 6 h of Cd2+ exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping