Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam
- Xu, D., Zhou, W., Peng, L.
- Biomedical Optics Express 8: 570-578 (Journal)
- Registered Authors
- Zhou, Weibin
- Imaging systems, Fluorescence microscopy, Spectroscopy, fluorescence and luminescence, Three-dimensional microscopy
- MeSH Terms
- 28270968 Full text @ Biomed. Opt. Express
Xu, D., Zhou, W., Peng, L. (2017) Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam. Biomedical Optics Express. 8:570-578.
Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes