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ZFIN ID: ZDB-PUB-170309-1
Exogenous gene integration mediated by genome editing technologies in zebrafish
Morita, H., Taimatsu, K., Yanagi, K., Kawahara, A.
Date: 2017
Source: Bioengineered 8(3): 287-295 (Review)
Registered Authors: Kawahara, Atsuo, Morita, Hitoshi
Keywords: CRISPR/Cas9, MMEJ, NHEJ, TALEN, genome editing, knock-in, zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified/genetics
  • CRISPR-Cas Systems/genetics*
  • Clustered Regularly Interspaced Short Palindromic Repeats/genetics*
  • Gene Editing/methods*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics*
PubMed: 28272984 Full text @ Bioengineered
Genome editing technologies, such as transcription activator-like effector nuclease (TALEN) and the clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated protein (Cas) systems, can induce DNA double-strand breaks (DSBs) at the targeted genomic locus, leading to frameshift-mediated gene disruption in the process of DSB repair. Recently, the technology-induced DSBs followed by DSB repairs are applied to integrate exogenous genes into the targeted genomic locus in various model organisms. In addition to a conventional knock-in technology mediated by homology-directed repair (HDR), novel knock-in technologies using refined donor vectors have also been developed with the genome editing technologies based on other DSB repair mechanisms, including non-homologous end joining (NHEJ) and microhomology-mediated end joining (MMEJ). Therefore, the improved knock-in technologies would contribute to freely modify the genome of model organisms.