PUBLICATION
Toxic effects of 56Fe ion radiation on the zebrafish (Danio rerio) embryonic development.
- Authors
- Si, J., Zhou, R., Song, J., Gan, L., Zhou, X., Di, C., Liu, Y., Mao, A., Zhao, Q., Wang, Y., Zhang, H.
- ID
- ZDB-PUB-170308-4
- Date
- 2017
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 186: 87-95 (Journal)
- Registered Authors
- Keywords
- (56)Fe ion radiation, Apoptosis, Developmental toxicity, Zebrafish embryos
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects
- Biological Assay
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects
- Embryonic Development/drug effects*
- Embryonic Development/genetics
- Embryonic Development/radiation effects*
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/radiation effects
- Iron/toxicity*
- Larva/drug effects
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Radiation, Ionizing*
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 28267650 Full text @ Aquat. Toxicol.
Citation
Si, J., Zhou, R., Song, J., Gan, L., Zhou, X., Di, C., Liu, Y., Mao, A., Zhao, Q., Wang, Y., Zhang, H. (2017) Toxic effects of 56Fe ion radiation on the zebrafish (Danio rerio) embryonic development.. Aquatic toxicology (Amsterdam, Netherlands). 186:87-95.
Abstract
All living organisms and ecosystems are permanently exposed to ionizing radiation. Of all the types of ionizing radiation, heavy ions such as 56Fe have the potential to cause the most severe biological effects. We therefore examined the effects and potential mechanisms of iron ion irradiation on the induction of developmental toxicity and apoptosis in zebrafish embryos. Zebrafish embryos at 4h post-fertilization (hpf) were divided into five groups: a control group; and four groups irradiated with 0.5, 1, 2, and 4Gy radiation, respectively. Mortality and teratogenesis were significantly increased, and spontaneous movement, heart rate, and swimming distance were decreased in the irradiated groups, accompanied by increased apoptosis. mRNA levels of genes involved in the apoptotic pathway, including p53, bax, bcl-2, and caspase-3, were significantly affected by radiation exposure. Moreover, protein expression levels of P53 and Bcl-2 changed in accordance with the corresponding mRNA expression levels. In addition, we detected the protein expression levels of γ-H2AX, which is a biomarker for radiation-induced DNA double-strand breaks, and found that γ-H2AX protein levels were significantly increased in the irradiated groups. Overall, the results of this study improve our understanding of the mechanisms of iron ion radiation-induced developmental toxicity and apoptosis, potentially involving the induction of DNA damage and mitochondrial dysfunction. The findings of this study may aid future impact assessment of environmental radioactivity in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping