PUBLICATION
Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells
- Authors
- Nguyen, P.D., Currie, P.D.
- ID
- ZDB-PUB-170302-7
- Date
- 2017
- Source
- Methods in molecular biology (Clifton, N.J.) 1556: 117-125 (Chapter)
- Registered Authors
- Currie, Peter D., Nguyen, Phong D.
- Keywords
- Live imaging, Muscle progenitor, Satellite cell, Skeletal muscle, Stem cell, Timelapse, Transgenics, Zebrafish
- MeSH Terms
-
- Muscle, Skeletal/cytology
- Satellite Cells, Skeletal Muscle/cytology
- Satellite Cells, Skeletal Muscle/metabolism
- Stem Cells/cytology*
- Stem Cells/metabolism*
- Biomarkers
- Phenotype
- Molecular Imaging/methods
- Animals
- Microscopy, Confocal
- Animals, Genetically Modified
- Zebrafish*
- Muscles/cytology*
- PubMed
- 28247347 Full text @ Meth. Mol. Biol.
Citation
Nguyen, P.D., Currie, P.D. (2017) Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells. Methods in molecular biology (Clifton, N.J.). 1556:117-125.
Abstract
Understanding muscle stem cell behaviors can potentially provide insights into how these cells act and respond during normal growth and diseased contexts. The zebrafish is an ideal model organism to examine these behaviors in vivo where it would normally be technically challenging in other mammalian models. This chapter will describe the procedures required to successfully conduct live imaging of zebrafish transgenics that has specifically been adapted for skeletal muscle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping