PUBLICATION
Sodium Selenite Prevents Paraquat-Induced Neurotoxicity in Zebrafish
- Authors
- Müller, T.E., Nunes, M.E., Menezes, C.C., Marins, A.T., Leitemperger, J., Gressler, A.C., Carvalho, F.B., de Freitas, C.M., Quadros, V.A., Fachinetto, R., Rosemberg, D.B., Loro, V.L.
- ID
- ZDB-PUB-170301-4
- Date
- 2017
- Source
- Molecular neurobiology 55(3): 1928-1941 (Journal)
- Registered Authors
- Keywords
- Behavior, Oxidative stress, Parkinson’s disease, Selenium, Zebrafish
- MeSH Terms
-
- Animals
- Herbicides/toxicity*
- Locomotion/drug effects
- Locomotion/physiology
- Neuroprotective Agents/administration & dosage*
- Oxidative Stress/drug effects*
- Oxidative Stress/physiology
- Paraquat/toxicity*
- Sodium Selenite/administration & dosage*
- Thiobarbituric Acid Reactive Substances/metabolism
- Zebrafish
- PubMed
- 28244005 Full text @ Mol. Neurobiol.
Citation
Müller, T.E., Nunes, M.E., Menezes, C.C., Marins, A.T., Leitemperger, J., Gressler, A.C., Carvalho, F.B., de Freitas, C.M., Quadros, V.A., Fachinetto, R., Rosemberg, D.B., Loro, V.L. (2017) Sodium Selenite Prevents Paraquat-Induced Neurotoxicity in Zebrafish. Molecular neurobiology. 55(3):1928-1941.
Abstract
Considering the antioxidant properties of sodium selenite (Na2SeO3) and the involvement of oxidative stress events in paraquat-induced neurotoxicity, this study investigated the protective effect of dietary Na2SeO3 on biochemical and behavioral parameters of zebrafish exposed to paraquat (PQ). Fish were pretreated with a Na2SeO3 diet for 21 days and then PQ (20 mg/kg) was administered intraperitoneally with six injections for 16 days. In the novel tank test, the Na2SeO3 diet prevented the locomotor impairments, as well as the increase in the time spent in the top area of the tank, and the exacerbation of freezing episodes. In the preference for conspecifics and in the mirror-induced aggression (MIA) tasks, Na2SeO3 prevented the increase in the latency to enter the area closer to conspecifics and the agonistic behavior of PQ-treated animals, respectively. Na2SeO3 prevented the increase of carbonylated protein (CP), reactive oxygen species (ROS), and nitrite/nitrate (NOx) levels, as well as the decrease in non-protein thiols (NPSH) levels. Regarding the antioxidant enzymatic defenses, Na2SeO3 prevented the increase in catalase (CAT) and glutathione peroxidase (GPx) activities caused by PQ. Altogether, dietary Na2SeO3 improves behavioral and biochemical function impaired by PQ treatment in zebrafish, by modulating not only redox parameters, but also anxiety- and aggressive-like phenotypes in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping