PUBLICATION
Morpholinos: Antisense and Sensibility
- Authors
- Blum, M., De Robertis, E.M., Wallingford, J.B., Niehrs, C.
- ID
- ZDB-PUB-170214-87
- Date
- 2015
- Source
- Developmental Cell 35: 145-9 (Review)
- Registered Authors
- De Robertis, Eddy
- Keywords
- none
- MeSH Terms
-
- Animals
- Developmental Biology/methods*
- Humans
- Morpholinos/genetics*
- Oligonucleotides, Antisense/genetics*
- Zebrafish/genetics
- Zebrafish/growth & development
- PubMed
- 26506304 Full text @ Dev. Cell
Citation
Blum, M., De Robertis, E.M., Wallingford, J.B., Niehrs, C. (2015) Morpholinos: Antisense and Sensibility. Developmental Cell. 35:145-9.
Abstract
For over 15 years, antisense morpholino oligonucleotides (MOs) have allowed developmental biologists to make key discoveries regarding developmental mechanisms in numerous model organisms. Recently, serious concerns have been raised as to the specificity of MO effects, and it has been recommended to discontinue their usage, despite the long experience of the scientific community with the MO tool in thousands of studies. Reviewing the many advantages afforded by MOs, we conclude that adequately controlled MOs should continue to be accepted as generic loss-of-function approach, as otherwise progress in developmental biology will greatly suffer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping