PUBLICATION
In vitro development of zebrafish vascular networks
- Authors
- Ibrahim, M., Richardson, M.K.
- ID
- ZDB-PUB-170214-6
- Date
- 2017
- Source
- Reproductive toxicology (Elmsford, N.Y.) 70: 102-115 (Journal)
- Registered Authors
- Richardson, Michael
- Keywords
- Angiogenesis, Embryoid bodies, Explant culture, Microfluidics, Vasculogenesis, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cells, Cultured
- Embryo, Nonmammalian/blood supply*
- Embryoid Bodies/physiology*
- Endothelial Cells/physiology*
- Green Fluorescent Proteins/genetics
- Heart/embryology
- Liver/cytology
- Liver/embryology
- Myocardium/cytology
- Neovascularization, Physiologic*
- Zebrafish
- PubMed
- 28192181 Full text @ Reprod. Toxicol.
Citation
Ibrahim, M., Richardson, M.K. (2017) In vitro development of zebrafish vascular networks. Reproductive toxicology (Elmsford, N.Y.). 70:102-115.
Abstract
A major limitation to culturing tissues and organs is the lack of a functional vascular network in vitro. The zebrafish possess many useful properties which makes it a promising model for such studies. Unfortunately, methods of culturing endothelial cells from this species are not well characterised. Here, we tried two methods (embryoid body culture and organ explants from transgenic zebrafish kdrl:GFP embryos) to develop in vitro vascular networks. In the kdrl:GFP line, endothelial cells expresses green fluorescent protein, which allows to track the vascular development in live cultures. We found that embryoid bodies showed significantly longer and wider branches of connected endothelial cells when grown in a microfluidic system than in static culture. Similarly, sprouting of kdrl:GFP+ cells from the tissue explants was observed in a 3D hydrogel matrix. This study is a step towards the development of zebrafish vascular networks in vitro.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping