PUBLICATION
Integrated in silico and experimental methods revealed that Arctigenin inhibited angiogenesis and HCT116 cell migration and invasion through regulating the H1F4A and Wnt/β-catenin pathway
- Authors
- Zhang, S., Li, J., Song, S., Li, J., Tong, R., Zang, Z., Jiang, Q., Cai, L.
- ID
- ZDB-PUB-170214-138
- Date
- 2015
- Source
- Molecular Biosystems 11: 2878-84 (Journal)
- Registered Authors
- Li, Jie
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Cell Movement*/drug effects
- Cell Proliferation/drug effects
- Computational Biology
- Computer Simulation*
- Furans/chemistry
- Furans/pharmacology
- Furans/therapeutic use*
- HCT116 Cells
- Histones/metabolism*
- Human Umbilical Vein Endothelial Cells/drug effects
- Human Umbilical Vein Endothelial Cells/metabolism
- Humans
- Lignans/chemistry
- Lignans/pharmacology
- Lignans/therapeutic use*
- Molecular Sequence Data
- Neoplasm Invasiveness
- Neovascularization, Pathologic/drug therapy*
- Protein Interaction Maps/drug effects
- Proteomics
- Wnt Signaling Pathway*/drug effects
- Wound Healing/drug effects
- Zebrafish/genetics
- PubMed
- 26267229 Full text @ Mol. Biosyst.
Citation
Zhang, S., Li, J., Song, S., Li, J., Tong, R., Zang, Z., Jiang, Q., Cai, L. (2015) Integrated in silico and experimental methods revealed that Arctigenin inhibited angiogenesis and HCT116 cell migration and invasion through regulating the H1F4A and Wnt/β-catenin pathway. Molecular Biosystems. 11:2878-84.
Abstract
Arctigenin (ARG) has been previously reported to exert diverse biological activities including anti-proliferation, anti-inflammatory, and antiviral, etc. In the current study, the anti-metastasis and anti-angiogenesis activities of ARG were investigated. To further understand how ARG played these bioactivities, proteomic approaches were used to profile the proteome changes in response to ARG treatment using 2DE-MS/MS. Using these approaches, a total of 50 differentially expressed proteins were identified and clustered. Bioinformatics analysis suggested that multiple signalling pathways were involved. Moreover, ARG induced anti-metastatic and anti-angiogenesis activities were mainly accompanied by a deactivation of the Wnt/β-catenin pathway in HCT116 cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping