Effects of cadmium, manganese and lead on locomotor activity and neurexin2a expression in zebrafish
- Tu, H., Fan, C., Chen, X., Liu, J., Wang, B., Huang, Z., Zhang, Y., Meng, X., Zou, F.
- Environmental toxicology and chemistry 36(8): 2147-2154 (Journal)
- Registered Authors
- Chen, Xiaohui, Huang, Zhibin, Zhang, Yiyue
- Locomotor activity, Metals, Neurexin, Zebrafish
- MeSH Terms
- Drug Synergism
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects
- Embryonic Development/genetics
- Larva/drug effects
- Locomotion/drug effects*
- 28120348 Full text @ Environ. Toxicol. Chem.
Tu, H., Fan, C., Chen, X., Liu, J., Wang, B., Huang, Z., Zhang, Y., Meng, X., Zou, F. (2017) Effects of cadmium, manganese and lead on locomotor activity and neurexin2a expression in zebrafish. Environmental toxicology and chemistry. 36(8):2147-2154.
The synaptic adhesion protein Neurexin 2a (Nrxn2a) plays a key role in neuronal development and is associated with cognitive functioning and locomotor behavior. Although low-level metal exposure poses a potential risk to the human nervous system, especially during the developmental stages, little is known about the effects of metal exposures on nrxn2a expression during embryonic development. We therefore exposed wild-type zebrafish embryos/larvae to cadmium (CdCl2 ), manganese (MnCl2 ), and lead ([CH3 COO]2 Pb), to determine their effect on mortality, malformation, and hatching rate. Concentrations of these metals in zebrafish were detected by inductively coupled plasma mass spectrometry analysis. Locomotor activity of zebrafish larvae was analyzed using a video-track tracking system. Expression of nrxn2a was assessed by in situ hybridization and quantitative polymerase chain reaction. The results showed that mortality, malformation, and bioaccumulation increased as the exposure dosages and duration increased. Developmental exposure to these metals significantly reduced larval swim distance and velocity. The nrxn2aa and nrxn2ab genes were expressed in the central nervous system and downregulated by almost all of the 3 metals, especially Pb. These data demonstrate that exposure to metals downregulates nrxn2a in the zebrafish model system, and this is likely linked to concurrent developmental processes. Environ Toxicol Chem 2017;36:2147-2154. © 2017 SETAC.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes