PUBLICATION
Negative effect of chronic cadmium exposure on growth, histology, ultrastructure, antioxidant and innate immune responses in the liver of zebrafish: Preventive role of blue light emitting diodes
- Authors
- Yuan, S.S., Lv, Z.M., Zhu, A.Y., Zheng, J.L., Wu, C.W.
- ID
- ZDB-PUB-170117-2
- Date
- 2017
- Source
- Ecotoxicology and environmental safety 139: 18-26 (Journal)
- Registered Authors
- Keywords
- Metal exposure, NF-κB, Non-specific immunity, Nrf2, Oxidative stress, Spectra
- MeSH Terms
-
- Animals
- Antioxidants*/metabolism
- Cadmium/toxicity*
- Down-Regulation
- Environmental Exposure
- Immunity, Innate*/drug effects
- Immunity, Innate*/genetics
- Light*
- Liver/drug effects*
- Liver/metabolism
- Liver/pathology
- Liver/ultrastructure
- NF-E2-Related Factor 2/genetics
- NF-E2-Related Factor 2/metabolism
- NF-kappa B/genetics
- NF-kappa B/metabolism
- Oxidative Stress*
- RNA, Messenger/metabolism
- Up-Regulation
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 28092736 Full text @ Ecotoxicol. Environ. Saf.
Citation
Yuan, S.S., Lv, Z.M., Zhu, A.Y., Zheng, J.L., Wu, C.W. (2017) Negative effect of chronic cadmium exposure on growth, histology, ultrastructure, antioxidant and innate immune responses in the liver of zebrafish: Preventive role of blue light emitting diodes. Ecotoxicology and environmental safety. 139:18-26.
Abstract
The present study explored the possible preventive effects of blue light emitting diodes (LEDs) on cadmium (Cd)-induced oxidative stress and immunotoxicity in zebrafish. To this end, zebrafish were exposed to a white fluorescent bulb or blue LEDs (LDB, peak at 450nm, at an irradiance of 0.9W/m2), and 0 or 30µgL-1 waterborne Cd for 5 weeks. Growth performance, survival rate, and hepatic histology, ultrastructure, antioxidant and innate immune responses were determined in zebrafish. Cd exposure alone reduced growth and survival rate, and induced oxidative damage and changes in histology and ultrastructure. However, Cd exposure in combination with LDB apparently relieved these negative effects. The alleviation of adverse effects might result from the up-regulation of antioxidant and innate immune genes at transcriptional, translational, or post-translational levels. Cd exposure alone dramatically enhanced mRNA levels of nuclear transcription factor κB (NF-κB) and E2-related factor (Nrf2). However, compared to Cd exposure alone, Cd exposure in combination with LDB apparently down-regulated both genes. Taken together, our results suggest that chronic Cd exposure induced a negative effect on zebrafish, possibly involved in NF-κB-induced immunotoxicity and Nrf2-induced oxidative stress. Finally, for the first time, our data demonstrated that LDB could protect fish against Cd toxicity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping