ZFIN ID: ZDB-PUB-170115-6
Wnt signaling balances specification of the cardiac and pharyngeal muscle fields
Mandal, A., Holowiecki, A., Song, Y.C., Waxman, J.S.
Date: 2017
Source: Mechanisms of Development   143: 32-41 (Journal)
Registered Authors: Waxman, Joshua
Keywords: Cardiac, Mesoderm patterning, Organ fields, Pharyngeal muscle, Wnt signaling, Zebrafish
MeSH Terms:
  • Actins/genetics
  • Actins/metabolism
  • Animals
  • Animals, Genetically Modified
  • Body Patterning/genetics*
  • Cytoskeletal Proteins/genetics*
  • Cytoskeletal Proteins/metabolism
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental*
  • Homeobox Protein Nkx-2.5/genetics
  • Homeobox Protein Nkx-2.5/metabolism
  • Mesoderm/cytology
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Myocardium/cytology
  • Myocardium/metabolism*
  • Pharyngeal Muscles/cytology
  • Pharyngeal Muscles/embryology
  • Pharyngeal Muscles/metabolism*
  • Signal Transduction
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Transcription Factor 7-Like 1 Protein/genetics
  • Transcription Factor 7-Like 1 Protein/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Wnt Proteins/genetics*
  • Wnt Proteins/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 28087459 Full text @ Mech. Dev.
Canonical Wnt/β-catenin (Wnt) signaling plays multiple conserved roles during fate specification of cardiac progenitors in developing vertebrate embryos. Although lineage analysis in ascidians and mice has indicated there is a close relationship between the cardiac second heart field (SHF) and pharyngeal muscle (PM) progenitors, the signals underlying directional fate decisions of the cells within the cardio-pharyngeal muscle field in vertebrates are not yet understood. Here, we examined the temporal requirements of Wnt signaling in cardiac and PM development. In contrast to a previous report in chicken embryos that suggested Wnt inhibits PM development during somitogenesis, we find that in zebrafish embryos Wnt signaling is sufficient to repress PM development during anterior-posterior patterning. Importantly, the temporal sensitivity of dorso-anterior PMs to increased Wnt signaling largely overlaps with when Wnt signaling promotes specification of the adjacent cardiac progenitors. Furthermore, we find that excess early Wnt signaling can cell autonomously promote expansion of the first heart field (FHF) progenitors at the expense of PM and SHF within the anterior lateral plate mesoderm (ALPM). Our study provides insight into an antagonistic developmental mechanism that balances the sizes of the adjacent cardiac and PM progenitor fields in early vertebrate embryos.