PUBLICATION
A Novel igf3 Gene in Common Carp (Cyprinus carpio): Evidence for Its Role in Regulating Gonadal Development
- Authors
- Song, F., Wang, L., Zhu, W., Fu, J., Dong, J., Dong, Z.
- ID
- ZDB-PUB-161222-4
- Date
- 2016
- Source
- PLoS One 11: e0168874 (Journal)
- Registered Authors
- Keywords
- Ovaries, Gonads, Zebrafish, Gene expression, Phylogenetic analysis, Polymerase chain reaction, Granulosa cells, Spermatids
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Carps/classification
- Carps/genetics*
- Carps/metabolism
- Estradiol/pharmacology
- Estrogens/pharmacology
- Female
- Fish Proteins/chemistry
- Fish Proteins/classification
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Gonads/drug effects
- Gonads/growth & development
- Gonads/metabolism*
- Male
- Ovary/drug effects
- Ovary/metabolism
- Ovary/pathology
- Phylogeny
- RNA, Messenger/chemistry
- RNA, Messenger/metabolism
- Sequence Alignment
- Somatomedins/chemistry
- Somatomedins/classification
- Somatomedins/genetics*
- Somatomedins/metabolism
- Testis/drug effects
- Testis/metabolism
- Testis/pathology
- Transcriptome/drug effects
- Zebrafish/genetics
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- PubMed
- 28002497 Full text @ PLoS One
Citation
Song, F., Wang, L., Zhu, W., Fu, J., Dong, J., Dong, Z. (2016) A Novel igf3 Gene in Common Carp (Cyprinus carpio): Evidence for Its Role in Regulating Gonadal Development. PLoS One. 11:e0168874.
Abstract
Since the insulin-like growth factor 3 (igf3) gene was recently discovered in fish ovary, its function in the gonads has received much attention. In this study, we isolated two igf3 subtypes from common carp (Cyprinus carpio), which comprised full-length cDNA of 707 and 1153 nucleotides encoding 205 and 198 amino acids (aa), respectively. The Igf3 aa sequence had the highest gene homology of 72% with the corresponding sequence in zebrafish (Danio rerio). Phylogenetic tree construction revealed that the C. carpio igf3 gene was first clustered with D. rerio and then with other teleost species. Igf3 mRNA was widely expressed, with expression being highest in the gonads and blood. In the gonad development stage, igf3a mRNA expression was highest in the maturity and recession stage of the ovary, and decline phase of the testis, while igf3b was highest in the recession and fully mature periods of the ovaries and testes, respectively. Western blotting of testis protein samples showed two bands of approximately 21 kDa and 34 kDa corresponding to the calculated molecular mass of the two Igf3 subtypes; no signal was detected in the ovary. The Igf3 protein was localized in the ovary granulosa cells and testis spermatogonium and spermatids. 17β-Ethinylestradiol treatment increased both ovary and testis igf3 mRNA expression. These findings suggest that Igf3 may play an important role in C. carpio gonadal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping