PUBLICATION

Regulator of G protein signaling 2 (Rgs2) regulates neural crest development through Pparδ-Sox10 cascade

Authors
Lin, S.J., Chiang, M.C., Shih, H.Y., Hsu, L.S., Yeh, T.H., Huang, Y.C., Lin, C.Y., Cheng, Y.C.
ID
ZDB-PUB-161217-11
Date
2017
Source
Biochimica et biophysica acta   1864(3): 463-474 (Journal)
Registered Authors
Keywords
Pparδ, Rgs2, Sox10, neural crest, zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Neural Crest/growth & development
  • Neural Crest/metabolism*
  • Neurogenesis/genetics*
  • PPAR delta/genetics*
  • PPAR delta/metabolism
  • Promoter Regions, Genetic
  • RGS Proteins/genetics*
  • RGS Proteins/metabolism
  • SOXE Transcription Factors/genetics*
  • SOXE Transcription Factors/metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcriptional Activation
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism
  • Wnt Signaling Pathway
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
27979767 Full text @ Biochim. Biophys. Acta
Abstract
Neural crest cells are multipotent progenitors that migrate extensively and differentiate into numerous derivatives. The developmental plasticity and migratory ability of neural crest cells render them an attractive model for studying numerous aspects of cell progression. We observed that zebrafish rgs2 was expressed in neural crest cells. Disrupting Rgs2 expression by using a dominant negative rgs2 construct or rgs2 morpholinos reduced GTPase-activating protein activity, induced the formation of neural crest progenitors, increased the proliferation of nonectomesenchymal neural crest cells, and inhibited the formation of ectomesenchymal neural crest derivatives. The transcription of pparda (which encodes Pparδ, a Wnt-activated transcription factor) was upregulated in Rgs2-deficient embryos, and Pparδ inhibition using a selective antagonist in the Rgs2-deficient embryos repaired neural crest defects. Our results clarify the mechanism through which the Rgs2-Pparδ cascade regulates neural crest development; specifically, Pparδ directly binds to the promoter and upregulates the transcription of the neural crest specifier sox10. This study reveals a unique regulatory mechanism, the Rgs2-Pparδ-Sox10 signaling cascade, and defines a key molecular regulator, Rgs2, in neural crest development.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes