|ZFIN ID: ZDB-PUB-161208-4|
Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis
Matsui, T., Bessho, Y.
|Source:||Methods in molecular biology (Clifton, N.J.) 1487: 367-378 (Chapter)|
|Registered Authors:||Bessho, Yasumasa, Matsui, Takaaki|
|Keywords:||FGF, Immunohistochemistry, MAPK, Quantitative analysis, Somite, Zebrafish|
|PubMed:||27924581 Full text @ Meth. Mol. Biol.|
Matsui, T., Bessho, Y. (2017) Analyzing ERK Signal Dynamics During Zebrafish Somitogenesis. Methods in molecular biology (Clifton, N.J.). 1487:367-378.
ABSTRACTDuring vertebrate development, Erk is activated and regulates multiple cellular processes such as cell growth, differentiation, migration, and adhesion in a spatiotemporal manner. Whole-mount immunohistochemistry using antibodies against diphosphorylated Erk (p-Erk; active form of Erk) is a very useful method for understanding the spatial and temporal patterns of Erk activity during embryonic development. However, the fixation step of this method stops embryo development at a certain time point, making it very difficult to observe and interpret Erk activity dynamics. In this chapter, we describe a strategy that combines immunohistochemistry and quantitative analyses of multiple fixed embryos to reconstruct Erk activity dynamics during zebrafish somitogenesis.
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