PUBLICATION

Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification

Authors
Ling, I.T., Rochard, L., Liao, E.C.
ID
ZDB-PUB-161203-3
Date
2017
Source
Developmental Biology   421(2): 219-232 (Journal)
Registered Authors
Liao, Eric
Keywords
Meckel's cartilage, Wnt, chondrocyte, endochondral ossification, osteoblast, zebrafish
MeSH Terms
  • Animals
  • Cartilage/metabolism
  • Cartilage/pathology
  • Cell Differentiation*
  • Cell Polarity
  • Cell Proliferation
  • Chondrocytes/cytology*
  • Chondrocytes/metabolism
  • Chondrogenesis*
  • Face
  • Gene Expression Regulation, Developmental
  • Joints/metabolism
  • Joints/pathology
  • Models, Biological
  • Muscles/metabolism
  • Muscles/pathology
  • Mutation/genetics
  • Osteogenesis*
  • Skull/metabolism
  • Time Factors
  • Wnt Proteins/metabolism*
  • Wnt Signaling Pathway
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
27908786 Full text @ Dev. Biol.
Abstract
Formation of the mandible requires progressive morphologic change, proliferation, differentiation and organization of chondrocytes preceding osteogenesis. The Wnt signaling pathway is involved in regulating bone development and maintenance. Chondrocytes that are fated to become bone require Wnt to polarize and orientate appropriately to initiate the endochondral ossification program. Although the canonical Wnt signaling has been well studied in the context of bone development, the effects of non-canonical Wnt signaling in regulating the timing of cartilage maturation and subsequent bone formation in shaping ventral craniofacial structure is not fully understood.. Here we examined the role of the non-canonical Wnt signaling pathway (wls, gpc4, wnt5b and wnt9a) in regulating zebrafish Meckel's cartilage maturation to the onset of osteogenic differentiation. We found that disruption of wls resulted in a significant loss of craniofacial bone, whereas lack of gpc4, wnt5b and wnt9a resulted in severely delayed endochondral ossification. This study demonstrates the importance of the non-canonical Wnt pathway in regulating coordinated ventral cartilage morphogenesis and ossification.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes