ZFIN ID: ZDB-PUB-161129-4
Internal epitope tagging informed by relative lack of sequence conservation
Burg, L., Zhang, K., Bonawitz, T., Grajevskaja, V., Bellipanni, G., Waring, R., Balciunas, D.
Date: 2016
Source: Scientific Reports   6: 36986 (Journal)
Registered Authors: Balciunas, Darius, Bellipanni, Gianfranco
Keywords: CRISPR-Cas9 genome editing, Genetic engineering, Zebrafish
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Antibodies/genetics
  • Conserved Sequence/genetics*
  • Epitopes/genetics*
  • Proteins/genetics
  • Sequence Alignment/methods
  • Zebrafish
PubMed: 27892520 Full text @ Sci. Rep.
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ABSTRACT
Many experimental techniques rely on specific recognition and stringent binding of proteins by antibodies. This can readily be achieved by introducing an epitope tag. We employed an approach that uses a relative lack of evolutionary conservation to inform epitope tag site selection, followed by integration of the tag-coding sequence into the endogenous locus in zebrafish. We demonstrate that an internal epitope tag is accessible for antibody binding, and that tagged proteins retain wild type function.
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