PUBLICATION
Use of Zebrafish Larvae as a Multi-Endpoint Platform to Characterize the Toxicity Profile of Silica Nanoparticles
- Authors
- Pham, D.H., De Roo, B., Nguyen, X.B., Vervaele, M., Kecskés, A., Ny, A., Copmans, D., Vriens, H., Locquet, J.P., Hoet, P., de Witte, P.A.
- ID
- ZDB-PUB-161123-7
- Date
- 2016
- Source
- Scientific Reports 6: 37145 (Journal)
- Registered Authors
- Keywords
- Developmental biology, Risk factors
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects
- Chemical and Drug Induced Liver Injury
- Embryo, Nonmammalian/drug effects*
- Endpoint Determination
- Heart/drug effects
- Nanoparticles/chemistry
- Nanoparticles/toxicity*
- Particle Size
- Silicon Dioxide/chemistry
- Silicon Dioxide/toxicity*
- Zebrafish
- PubMed
- 27872490 Full text @ Sci. Rep.
Citation
Pham, D.H., De Roo, B., Nguyen, X.B., Vervaele, M., Kecskés, A., Ny, A., Copmans, D., Vriens, H., Locquet, J.P., Hoet, P., de Witte, P.A. (2016) Use of Zebrafish Larvae as a Multi-Endpoint Platform to Characterize the Toxicity Profile of Silica Nanoparticles. Scientific Reports. 6:37145.
Abstract
Nanomaterials are being extensively produced and applied in society. Human and environmental exposures are, therefore, inevitable and so increased attention is being given to nanotoxicity. While silica nanoparticles (NP) are one of the top five nanomaterials found in consumer and biomedical products, their toxicity profile is poorly characterized. In this study, we investigated the toxicity of silica nanoparticles with diameters 20, 50 and 80 nm using an in vivo zebrafish platform that analyzes multiple endpoints related to developmental, cardio-, hepato-, and neurotoxicity. Results show that except for an acceleration in hatching time and alterations in the behavior of zebrafish embryos/larvae, silica NPs did not elicit any developmental defects, nor any cardio- and hepatotoxicity. The behavioral alterations were consistent for both embryonic photomotor and larval locomotor response and were dependent on the concentration and the size of silica NPs. As embryos and larvae exhibited a normal touch response and early hatching did not affect larval locomotor response, the behavior changes observed are most likely the consequence of modified neuroactivity. Overall, our results suggest that silica NPs do not cause any developmental, cardio- or hepatotoxicity, but they pose a potential risk for the neurobehavioral system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping