PUBLICATION
Ion transport in the zebrafish kidney from a human disease angle: possibilities, considerations and future perspectives
- Authors
- Kersten, S., Arjona, F.J.
- ID
- ZDB-PUB-161118-5
- Date
- 2017
- Source
- American journal of physiology. Renal physiology 312(1): F172-F189 (Review)
- Registered Authors
- Arjona, F.J.
- Keywords
- Human disease, Ion reabsorption, Kidney, Translational value, Zebrafish
- MeSH Terms
-
- Animals
- Ion Transport/genetics*
- Ion Transport/physiology*
- Kidney/metabolism*
- Kidney Diseases/genetics
- Kidney Diseases/metabolism*
- Membrane Transport Proteins/metabolism*
- Models, Animal
- Nephrons/metabolism*
- Zebrafish/genetics
- PubMed
- 27852607 Full text @ Am. J. Physiol. Renal Physiol.
Citation
Kersten, S., Arjona, F.J. (2017) Ion transport in the zebrafish kidney from a human disease angle: possibilities, considerations and future perspectives. American journal of physiology. Renal physiology. 312(1):F172-F189.
Abstract
Unique experimental advantages, such as its embryonic/larval transparency, high-throughput nature and ease of genetic modification, underpin the rapid emergence of the zebrafish (Danio rerio) as a preeminent model in biomedical research. Particularly in the field of nephrology, the zebrafish provides a promising model for studying the physiological implications of human solute transport processes along consecutive nephron segments. However, while the zebrafish might be considered a valuable model for numerous renal ion transport diseases and functional studies of many channels and transporters, not all human renal electrolyte transport mechanisms and human diseases can be modeled in the zebrafish. With this review, we explore the ontogeny of zebrafish renal ion transport, its nephron structure and function and thereby demonstrate the clinical translational value of this model. By critical assessment of genomic and amino acid conservation of human proteins involved in renal ion handling (channels, transporters and claudins), kidney and nephron segment conservation, and renal electrolyte transport physiology in the zebrafish, we provide researchers and nephrologists with an indication of the possibilities and considerations of the zebrafish as a model for human renal ion transport. Combined with advanced techniques envisioned for the future, implementation of the zebrafish might expand beyond unraveling pathophysiological mechanisms that underlie distinct genetic or environmentally, i.e. pharmacological and life-style, induced renal transport deficits. Specifically, the ease of drug administration and the exploitation of improved genetic approaches might argue for the adoption of the zebrafish as model for preclinical personalized medicine for distinct renal diseases and renal electrolyte transport proteins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping