PUBLICATION
Mac Protein is not an Essential Virulence Factor for the Virulent Reference Strain Streptococcus suis P1/7
- Authors
- Xiao, G., Wu, Z., Zhang, S., Tang, H., Wang, F., Lu, C.
- ID
- ZDB-PUB-161117-4
- Date
- 2017
- Source
- Current microbiology 74(1): 90-96 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism*
- Female
- Immunoglobulin M/immunology
- Macrophages/immunology
- Macrophages/microbiology
- Mice
- Mice, Inbred BALB C
- Phagocytosis
- RAW 264.7 Cells
- Streptococcal Infections/immunology
- Streptococcal Infections/microbiology
- Streptococcal Infections/veterinary*
- Streptococcus suis/genetics
- Streptococcus suis/metabolism
- Streptococcus suis/pathogenicity*
- Swine
- Swine Diseases/immunology
- Swine Diseases/microbiology*
- Virulence
- Virulence Factors/genetics
- Virulence Factors/metabolism*
- Zebrafish
- PubMed
- 27847975 Full text @ Curr. Microbiol.
Citation
Xiao, G., Wu, Z., Zhang, S., Tang, H., Wang, F., Lu, C. (2017) Mac Protein is not an Essential Virulence Factor for the Virulent Reference Strain Streptococcus suis P1/7. Current microbiology. 74(1):90-96.
Abstract
Streptococcus suis is a major pathogen of pigs and also an important zoonotic agent for humans. A S. suis protein containing Mac-1 domain (designated Mac) is a protective antigen, exclusively cleaves porcine IgM, and contributes to complement evasion with the presence of high titers of specific porcine anti-S. suis IgM, but its role in S. suis virulence has not been investigated in natural healthy host without specific IgM. In this study, a mac deletion mutant was constructed by homologous recombination in S. suis serotype 2 virulent reference strain P1/7. Deletion of mac did not significantly influence phagocytosis or intracellular survival within murine macrophages RAW264.7, or the oxidative-burst induction of RAW264.7 and murine neutrophils. Furthermore, the mutant is as virulent as the wild-type strain in pig, mouse, and zebrafish infection models. Our data suggest that Mac is not essential for S. suis virulence in strain P1/7 in natural healthy host without specific IgM, and the immunogenicity of Mac does not appear to correlate with its significance for virulence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping