PUBLICATION
Manipulation of zebrafish's orientation using artificial cilia in a microchannel with actively adaptive wall design
- Authors
- Mani, K., Chang Chien, T.C., Panigrahi, B., Chen, C.Y.
- ID
- ZDB-PUB-161110-18
- Date
- 2016
- Source
- Scientific Reports 6: 36385 (Journal)
- Registered Authors
- Keywords
- Biomedical engineering, Mechanical engineering
- MeSH Terms
-
- Animals
- Behavior, Animal
- Cilia/physiology
- Microfluidic Analytical Techniques/instrumentation*
- Models, Animal
- Orientation, Spatial
- Zebrafish/physiology*
- PubMed
- 27821862 Full text @ Sci. Rep.
Citation
Mani, K., Chang Chien, T.C., Panigrahi, B., Chen, C.Y. (2016) Manipulation of zebrafish's orientation using artificial cilia in a microchannel with actively adaptive wall design. Scientific Reports. 6:36385.
Abstract
The zebrafish is a powerful genetic model organism especially in the biomedical chapter for new drug discovery and development. The genetic toolbox which this vertebrate possesses opens a new window to investigate the etiology of human diseases with a high degree genetic similarity. Still, the requirements of laborious and time-consuming of contemporary zebrafish processing assays limit the procedure in carrying out such genetic screen at high throughput. Here, a zebrafish control scheme was initiated which includes the design and validation of a microfluidic platform to significantly increase the throughput and performance of zebrafish larvae manipulation using the concept of artificial cilia actuation. A moving wall design was integrated into this microfluidic platform first time in literature to accommodate zebrafish inside the microchannel from 1 day post-fertilization (dpf) to 6 dpf and can be further extended to 9 dpf for axial orientation control in a rotational range between 0 to 25 degrees at the minimum step of 2-degree increment in a stepwise manner. This moving wall feature was performed through the deflection of shape memory alloy wire embedded inside the microchannel controlled by the electrical waveforms with high accuracy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping