ZFIN ID: ZDB-PUB-161105-15
Independent modes of ganglion cell translocation ensure correct lamination of the zebrafish retina
Icha, J., Kunath, C., Rocha-Martins, M., Norden, C.
Date: 2016
Source: The Journal of cell biology   215: 259-275 (Journal)
Registered Authors: Icha, Jaroslav, Norden, Caren
Keywords: none
MeSH Terms:
  • Actin-Related Protein 2-3 Complex/metabolism
  • Animals
  • Cell Differentiation
  • Cell Movement*
  • Cell Nucleus/metabolism
  • Cell Survival
  • Embryo, Nonmammalian/cytology
  • Kinetics
  • Microtubules/metabolism
  • Models, Biological
  • Organelles/metabolism
  • Retinal Ganglion Cells/cytology*
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Zebrafish/embryology
  • Zebrafish/metabolism*
PubMed: 27810916 Full text @ J. Cell Biol.
ABSTRACT
The arrangement of neurons into distinct layers is critical for neuronal connectivity and function. During development, most neurons move from their birthplace to the appropriate layer, where they polarize. However, kinetics and modes of many neuronal translocation events still await exploration. In this study, we investigate retinal ganglion cell (RGC) translocation across the embryonic zebrafish retina. After completing their translocation, RGCs establish the most basal retinal layer where they form the optic nerve. Using in toto light sheet microscopy, we show that somal translocation of RGCs is a fast and directed event. It depends on basal process attachment and stabilized microtubules. Interestingly, interference with somal translocation induces a switch to multipolar migration. This multipolar mode is less efficient but still leads to successful RGC layer formation. When both modes are inhibited though, RGCs fail to translocate and induce lamination defects. This indicates that correct RGC translocation is crucial for subsequent retinal lamination.
ADDITIONAL INFORMATION