PUBLICATION
Proteomics Identification of Potential Candidates Involved in Cell Proliferation for Early Stage of Brain Regeneration in the Adult Zebrafish
- Authors
- Lim, F.T., Ogawa, S., Smith, A.I., Parhar, I.S.
- ID
- ZDB-PUB-161101-8
- Date
- 2017
- Source
- Zebrafish 14(1): 10-22 (Journal)
- Registered Authors
- Ogawa, Satoshi
- Keywords
- BrdU, DIGE, habenula, neurogenesis
- MeSH Terms
-
- Animals
- Biomarkers/metabolism
- Brain/cytology
- Brain/physiology*
- Cell Proliferation/physiology*
- Electrophoresis, Gel, Two-Dimensional
- Proteomics/methods*
- Regeneration
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Tandem Mass Spectrometry
- Zebrafish/growth & development
- Zebrafish/physiology*
- PubMed
- 27797681 Full text @ Zebrafish
Citation
Lim, F.T., Ogawa, S., Smith, A.I., Parhar, I.S. (2017) Proteomics Identification of Potential Candidates Involved in Cell Proliferation for Early Stage of Brain Regeneration in the Adult Zebrafish. Zebrafish. 14(1):10-22.
Abstract
The central nervous system (CNS) of the non-mammalian vertebrates has better neuroregenerative capability as compared with the mammalian CNS. Regeneration of habenula was observed 40 days after damage in zebrafish. During the early stage of regeneration, we found a significant increase of apoptotic cells on day-1 post-damage and of proliferative cells on day-3 post-damage. To identify the molecular factor(s) involved in the early stages of neuroregeneration, differentially expressed proteins during sham, 20- and 40-h post-habenula damage were investigated by proteomic approach by using two-dimensional differential gel electrophoresis (2D-DIGE) coupled with Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight (MALDI-ToF) and tandem mass spectrometry. Protein profiles revealed 17 differentially (>1.5-fold) expressed proteins: 10 upregulated, 4 downregulated, 2 proteins were found to be downregulated at the early stage but upregulated at a later stage, and 1 protein was found to be upregulated at 2 different time points. All proteins identified can be summarized under few molecular processes involved in the early stages of neuroregeneration in zebrafish CNS: apoptosis regulation (Wnt inhibitory factor 1 [WIF1]), neuroprotection (metallothionein), cell proliferation (Spred2, ependymin, Lhx1, and Wnts), differentiation (Spred2, Lhx9, and Wnts), and morphogenesis (cytoplasmic actins and draculin). These protein profiling results suggest that drastic molecular changes occur in the neuroregenerative process during this period, which includes cell proliferation, differentiation, and protection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping