PUBLICATION
N-Cadherin is Involved in Neuronal Activity-Dependent Regulation of Myelinating Capacity of Zebrafish Individual Oligodendrocytes In Vivo
- Authors
- Chen, M., Xu, Y., Huang, R., Huang, Y., Ge, S., Hu, B.
- ID
- ZDB-PUB-161025-9
- Date
- 2016
- Source
- Molecular neurobiology 54(9): 6917-6930 (Journal)
- Registered Authors
- Hu, Bing
- Keywords
- In vivo, Myelinating capacity, N-cadherin, Neuronal activity, Single-cell electroporation, Zebrafish oligodendrocytes
- MeSH Terms
-
- Animals
- Axons/metabolism
- Cadherins/metabolism*
- Cyclic AMP/metabolism
- Models, Biological
- Molecular Imaging
- Myelin Sheath/metabolism*
- Neurons/metabolism*
- Oligodendroglia/metabolism*
- Pentylenetetrazole
- Peptides/pharmacology
- Potassium Channels, Inwardly Rectifying/metabolism
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism*
- PubMed
- 27771903 Full text @ Mol. Neurobiol.
Citation
Chen, M., Xu, Y., Huang, R., Huang, Y., Ge, S., Hu, B. (2016) N-Cadherin is Involved in Neuronal Activity-Dependent Regulation of Myelinating Capacity of Zebrafish Individual Oligodendrocytes In Vivo. Molecular neurobiology. 54(9):6917-6930.
Abstract
Stimulating neuronal activity increases myelin sheath formation by individual oligodendrocytes, but how myelination is regulated by neuronal activity in vivo is still not fully understood. While in vitro studies have revealed the important role of N-cadherin in myelination, our understanding in vivo remains quite limited. To obtain the role of N-cadherin during activity-dependent regulation of myelinating capacity of individual oligodendrocytes, we successfully built an in vivo dynamic imaging model of the Mauthner cell at the subcellular structure level in the zebrafish central nervous system. Enhanced green fluorescent protein (EGFP)-tagged N-cadherin was used to visualize the stable accumulations and mobile transports of N-cadherin by single-cell electroporation at the single-cell level. We found that pentylenetetrazol (PTZ) significantly enhanced the accumulation of N-cadherin in Mauthner axons, a response that was paralleled by enhanced sheath number per oligodendrocytes. By offsetting this phenotype using oligopeptide (AHAVD) which blocks the function of N-cadherin, we showed that PTZ regulates myelination in an N-cadherin-dependent manner. What is more, we further suggested that PTZ influences N-cadherin and myelination via a cAMP pathway. Consequently, our data indicated that N-cadherin is involved in neuronal activity-dependent regulation of myelinating capacity of zebrafish individual oligodendrocytes in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping