ZFIN ID: ZDB-PUB-160930-11
Renoprotective effect of erythropoietin in zebrafish after administration of gentamicin: an immunohistochemical study for β-catenin and c-kit expression
Cernaro, V., Sfacteria, A., Rifici, C., Macrì, F., Maricchiolo, G., Lacquaniti, A., Ricciardi, C.A., Buemi, A., Costantino, G., Santoro, D., Buemi, M.
Date: 2017
Source: Journal of nephrology   30(3): 385-391 (Journal)
Registered Authors:
Keywords: Erythropoietin, Gentamicin, Tubule regeneration, Zebrafish, c-Kit, β-Catenin
MeSH Terms:
  • Animals
  • Cell Proliferation/drug effects
  • Cytoprotection
  • Disease Models, Animal
  • Epoetin Alfa/pharmacology*
  • Gentamicins*
  • Immunohistochemistry*
  • Kidney Diseases/chemically induced
  • Kidney Diseases/drug therapy*
  • Kidney Diseases/metabolism
  • Kidney Diseases/pathology
  • Kidney Tubules/drug effects*
  • Kidney Tubules/metabolism
  • Kidney Tubules/pathology
  • Protective Agents/pharmacology*
  • Regeneration/drug effects*
  • Stem Cell Factor/metabolism*
  • Time Factors
  • Zebrafish
  • Zebrafish Proteins/metabolism*
  • beta Catenin/metabolism*
PubMed: 27679401 Full text @ J. Nephrol.
Gentamicin is an aminoglycoside antibiotic widely used in the treatment of infections caused by Gram-negative bacteria. The main limitation to its therapeutic effectiveness is the potential nephrotoxicity. Erythropoietin has a tissue protective effect widely demonstrated in the kidney. The aim of the present study was to evaluate the renoprotective effects of erythropoietin in a model of zebrafish (Danio rerio) after administration of gentamicin.
Sixty adult zebrafish were subdivided into three groups: group A was treated with gentamicin; group B received gentamicin and, 24 h later, epoetin alpha; group C received drug diluent only. In order to analyze the renoprotective activity of erythropoietin, the expression of c-kit and β-catenin was evaluated by immunohistochemistry.
Generally, the zebrafish renal tubule regenerates 15 days after an injury. Conversely, 7 days after gentamicin administration, animals treated with erythropoietin (group B) showed a better renal injury repair as documented by: increased expression of β-catenin, less degenerated tubules, greater number of centers of regeneration, positivity for c-kit only in immature-looking tubules and lymphohematopoietic cells.
The expression of c-kit and β-catenin suggests that erythropoietin may exert a role in regeneration reducing the extent of tubular damage from the outset after gentamicin administration.