|ZFIN ID: ZDB-PUB-160930-11|
Renoprotective effect of erythropoietin in zebrafish after administration of gentamicin: an immunohistochemical study for β-catenin and c-kit expression
Cernaro, V., Sfacteria, A., Rifici, C., Macrì, F., Maricchiolo, G., Lacquaniti, A., Ricciardi, C.A., Buemi, A., Costantino, G., Santoro, D., Buemi, M.
|Source:||Journal of nephrology 30(3): 385-391 (Journal)|
|Keywords:||Erythropoietin, Gentamicin, Tubule regeneration, Zebrafish, c-Kit, β-Catenin|
|PubMed:||27679401 Full text @ J. Nephrol.|
Cernaro, V., Sfacteria, A., Rifici, C., Macrì, F., Maricchiolo, G., Lacquaniti, A., Ricciardi, C.A., Buemi, A., Costantino, G., Santoro, D., Buemi, M. (2017) Renoprotective effect of erythropoietin in zebrafish after administration of gentamicin: an immunohistochemical study for β-catenin and c-kit expression. Journal of nephrology. 30(3):385-391.
Background Gentamicin is an aminoglycoside antibiotic widely used in the treatment of infections caused by Gram-negative bacteria. The main limitation to its therapeutic effectiveness is the potential nephrotoxicity. Erythropoietin has a tissue protective effect widely demonstrated in the kidney. The aim of the present study was to evaluate the renoprotective effects of erythropoietin in a model of zebrafish (Danio rerio) after administration of gentamicin.
Methods Sixty adult zebrafish were subdivided into three groups: group A was treated with gentamicin; group B received gentamicin and, 24 h later, epoetin alpha; group C received drug diluent only. In order to analyze the renoprotective activity of erythropoietin, the expression of c-kit and β-catenin was evaluated by immunohistochemistry.
Results Generally, the zebrafish renal tubule regenerates 15 days after an injury. Conversely, 7 days after gentamicin administration, animals treated with erythropoietin (group B) showed a better renal injury repair as documented by: increased expression of β-catenin, less degenerated tubules, greater number of centers of regeneration, positivity for c-kit only in immature-looking tubules and lymphohematopoietic cells.
Conclusion The expression of c-kit and β-catenin suggests that erythropoietin may exert a role in regeneration reducing the extent of tubular damage from the outset after gentamicin administration.
- Antibodies (2)