PUBLICATION
Renoprotective effect of erythropoietin in zebrafish after administration of gentamicin: an immunohistochemical study for β-catenin and c-kit expression
- Authors
- Cernaro, V., Sfacteria, A., Rifici, C., Macrì, F., Maricchiolo, G., Lacquaniti, A., Ricciardi, C.A., Buemi, A., Costantino, G., Santoro, D., Buemi, M.
- ID
- ZDB-PUB-160930-11
- Date
- 2017
- Source
- Journal of nephrology 30(3): 385-391 (Journal)
- Registered Authors
- Keywords
- Erythropoietin, Gentamicin, Tubule regeneration, Zebrafish, c-Kit, β-Catenin
- MeSH Terms
-
- Zebrafish Proteins/metabolism*
- Gentamicins*
- Cytoprotection
- Animals
- Time Factors
- beta Catenin/metabolism*
- Immunohistochemistry*
- Disease Models, Animal
- Epoetin Alfa/pharmacology*
- Zebrafish
- Kidney Tubules/drug effects*
- Kidney Tubules/metabolism
- Kidney Tubules/pathology
- Protective Agents/pharmacology*
- Kidney Diseases/chemically induced
- Kidney Diseases/drug therapy*
- Kidney Diseases/metabolism
- Kidney Diseases/pathology
- Regeneration/drug effects*
- Stem Cell Factor/metabolism*
- Cell Proliferation/drug effects
- PubMed
- 27679401 Full text @ J. Nephrol.
Citation
Cernaro, V., Sfacteria, A., Rifici, C., Macrì, F., Maricchiolo, G., Lacquaniti, A., Ricciardi, C.A., Buemi, A., Costantino, G., Santoro, D., Buemi, M. (2017) Renoprotective effect of erythropoietin in zebrafish after administration of gentamicin: an immunohistochemical study for β-catenin and c-kit expression. Journal of nephrology. 30(3):385-391.
Abstract
Background Gentamicin is an aminoglycoside antibiotic widely used in the treatment of infections caused by Gram-negative bacteria. The main limitation to its therapeutic effectiveness is the potential nephrotoxicity. Erythropoietin has a tissue protective effect widely demonstrated in the kidney. The aim of the present study was to evaluate the renoprotective effects of erythropoietin in a model of zebrafish (Danio rerio) after administration of gentamicin.
Methods Sixty adult zebrafish were subdivided into three groups: group A was treated with gentamicin; group B received gentamicin and, 24 h later, epoetin alpha; group C received drug diluent only. In order to analyze the renoprotective activity of erythropoietin, the expression of c-kit and β-catenin was evaluated by immunohistochemistry.
Results Generally, the zebrafish renal tubule regenerates 15 days after an injury. Conversely, 7 days after gentamicin administration, animals treated with erythropoietin (group B) showed a better renal injury repair as documented by: increased expression of β-catenin, less degenerated tubules, greater number of centers of regeneration, positivity for c-kit only in immature-looking tubules and lymphohematopoietic cells.
Conclusion The expression of c-kit and β-catenin suggests that erythropoietin may exert a role in regeneration reducing the extent of tubular damage from the outset after gentamicin administration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping