PUBLICATION
The coiled-coil domain of zebrafish TRPM7 regulates Mg·nucleotide sensitivity
- Authors
- Jansen, C., Sahni, J., Suzuki, S., Horgen, F.D., Penner, R., Fleig, A.
- ID
- ZDB-PUB-160916-8
- Date
- 2016
- Source
- Scientific Reports 6: 33459 (Journal)
- Registered Authors
- Keywords
- Experimental models of disease, Ion transport
- MeSH Terms
-
- Adenosine Triphosphate/pharmacology*
- Animals
- Biophysical Phenomena
- Boron Compounds/pharmacology
- Cell Proliferation/drug effects
- Chickens
- Electric Conductivity
- HEK293 Cells
- Humans
- Hydrogen-Ion Concentration
- Liver/metabolism
- Magnesium/pharmacology
- Mutant Proteins/pharmacology
- Osmolar Concentration
- Protein Domains
- Protein Serine-Threonine Kinases/chemistry*
- Protein Serine-Threonine Kinases/metabolism*
- Structure-Activity Relationship
- TRPM Cation Channels/chemistry*
- TRPM Cation Channels/metabolism*
- Zebrafish/metabolism*
- Zebrafish Proteins/chemistry*
- Zebrafish Proteins/metabolism*
- PubMed
- 27628598 Full text @ Sci. Rep.
Citation
Jansen, C., Sahni, J., Suzuki, S., Horgen, F.D., Penner, R., Fleig, A. (2016) The coiled-coil domain of zebrafish TRPM7 regulates Mg·nucleotide sensitivity. Scientific Reports. 6:33459.
Abstract
TRPM7 is a member of the Transient-Receptor-Potential Melastatin ion channel family. TRPM7 is a unique fusion protein of an ion channel and an α-kinase. Although mammalian TRPM7 is well characterized biophysically and its pivotal role in cancer, ischemia and cardiovascular disease is becoming increasingly evident, the study of TRPM7 in mouse models has been hampered by embryonic lethality of transgenic ablations. In zebrafish, functional loss of TRPM7 (drTRPM7) manifests itself in an array of non-lethal physiological malfunctions. Here, we investigate the regulation of wild type drTRPM7 and multiple C-terminal truncation mutants. We find that the biophysical properties of drTRPM7 are very similar to mammalian TRPM7. However, pharmacological profiling reveals that drTRPM7 is facilitated rather than inhibited by 2-APB, and that the TRPM7 inhibitor waixenicin A has no effect. This is reminiscent of the pharmacological profile of human TRPM6, the sister channel kinase of TRPM7. Furthermore, using truncation mutations, we show that the coiled-coil domain of drTRPM7 is involved in the channel's regulation by magnesium (Mg) and Mg·adenosine triphosphate (Mg·ATP). We propose that drTRPM7 has two protein domains that regulate inhibition by intracellular magnesium and nucleotides, and one domain that is concerned with sensing magnesium only.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping