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ZIRC
ZFIN ID: ZDB-PUB-160902-10
Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos
Tseng, L.C., Tang, C.H., Jiang, Y.J.
Date: 2016
Source: Methods in molecular biology (Clifton, N.J.)   1470: 213-24 (Chapter)
Registered Authors: Jiang, Yun-Jin, Tang, Chih-Hao, Tseng, Li-Chuan
Keywords: In situ hybridization, Knockdown, Morpholinos, RNA probe, Zebrafish
MeSH Terms:
  • Animals
  • Embryo, Nonmammalian
  • Gastrulation/genetics
  • Gene Expression Regulation, Developmental*
  • Gene Knockdown Techniques/methods
  • High-Throughput Screening Assays/methods*
  • In Situ Hybridization/methods*
  • Microinjections/methods
  • Morpholinos/genetics*
  • Morpholinos/pharmacology
  • Ubiquitin-Specific Proteases/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
PubMed: 27581296 Full text @ Meth. Mol. Biol.
ABSTRACT
High-throughput screening with a loss-of-function strategy is a logical and efficient way to identify novel genes involved in biological processes of interest. In zebrafish, morpholinos have been developed as a convenient tool to knock down gene expression. Here, we describe procedures for systematic screening using morpholinos in zebrafish to identify novel deubiquitylases involved in convergent extension during gastrulation. In this example, we examine candidates based on embryonic morphology and molecular signals of whole mount in situ hybridization assay.
ADDITIONAL INFORMATION No data available