PUBLICATION
A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis
- Authors
- Thambyrajah, R., Ucanok, D., Jalali, M., Hough, Y., Wilkinson, R.N., McMahon, K., Moore, C., Gering, M.
- ID
- ZDB-PUB-160721-5
- Date
- 2016
- Source
- Developmental Biology 417(1): 25-39 (Journal)
- Registered Authors
- Gering, Martin, Moore, Chris, Ucanok, Deniz, Wilkinson, Robert
- Keywords
- Endothelial to haematopoietic transition, Gene trap, Gfi1, Haematopoiesis, Haemogenic endothelium, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Base Sequence
- Cell Differentiation
- Core Binding Factor Alpha 2 Subunit/biosynthesis
- DNA Transposable Elements/genetics*
- DNA-Binding Proteins/biosynthesis*
- DNA-Binding Proteins/genetics
- Erythrocytes/cytology*
- Erythrocytes/metabolism
- Erythropoiesis/genetics*
- Gene Expression Regulation, Developmental
- Green Fluorescent Proteins/genetics
- Hematopoietic Stem Cells/cytology*
- Promoter Regions, Genetic/genetics
- RNA, Messenger/biosynthesis
- Receptors, Notch/genetics
- Receptors, Notch/metabolism
- Vascular Endothelial Growth Factor A/genetics
- Vascular Endothelial Growth Factor A/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- PubMed
- 27432513 Full text @ Dev. Biol.
Citation
Thambyrajah, R., Ucanok, D., Jalali, M., Hough, Y., Wilkinson, R.N., McMahon, K., Moore, C., Gering, M. (2016) A gene trap transposon eliminates haematopoietic expression of zebrafish Gfi1aa, but does not interfere with haematopoiesis. Developmental Biology. 417(1):25-39.
Abstract
A transposon-mediated gene trap screen identified the zebrafish line qmc551 that expresses a GFP reporter in primitive erythrocytes and also in haemogenic endothelial cells, which give rise to haematopoietic stem and progenitor cells (HSPCs) that seed sites of larval and adult haematopoiesis. The transposon that mediates this GFP expression is located in intron 1 of the gfi1aa gene, one of three zebrafish paralogs that encode transcriptional repressors homologous to mammalian Gfi1 and Gfi1b proteins. In qmc551 transgenics, GFP expression is under the control of the endogenous gfi1aa promoter, recapitulates early gfi1aa expression and allows live observation of gfi1aa promoter activity. While the transposon integration interferes with the expression of gfi1aa mRNA in haematopoietic cells, homozygous qmc551 fish are viable and fertile, and display normal primitive and definitive haematopoiesis. Retained expression of Gfi1b in primitive erythrocytes and up-regulation of Gfi1ab at the onset of definitive haematopoiesis in homozygous qmc551 carriers, are sufficient to allow normal haematopoiesis. This finding contradicts previously published morpholino data that suggested an essential role for zebrafish Gfi1aa in primitive erythropoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping