PUBLICATION

Embryonic atrazine exposure elicits alterations in genes associated with neuroendocrine function in adult male zebrafish

Authors
Wirbisky, S.E., SepĂșlveda, M.S., Weber, G.J., Jannasch, A.S., Horzmann, K.A., Freeman, J.L.
ID
ZDB-PUB-160716-18
Date
2016
Source
Toxicological sciences : an official journal of the Society of Toxicology   153(1): 149-64 (Journal)
Registered Authors
Freeman, Jennifer
Keywords
atrazine, developmental origins of health and disease, hormones, neuroendocrine system, transcriptomics, zebrafish
Datasets
GEO:GSE72242, GEO:GSE72244, GEO:GSE72243
MeSH Terms
  • Animals
  • Atrazine/toxicity*
  • Brain/drug effects
  • Brain/metabolism
  • Endocrine Disruptors/toxicity*
  • Gene Expression Regulation, Developmental/drug effects*
  • Herbicides/toxicity*
  • Male
  • Neurosecretory Systems/drug effects*
  • Real-Time Polymerase Chain Reaction
  • Testis/drug effects
  • Testis/metabolism
  • Transcriptome
  • Zebrafish/embryology*
PubMed
27413107 Full text @ Toxicol. Sci.
Abstract
The developmental origins of health and disease (DOHaD) hypothesis states that exposure to environmental stressors early in life can elicit genome and epigenome changes resulting in an increased susceptibility of a disease state during adulthood. Atrazine, a common agricultural herbicide used throughout the Midwestern United States, frequently contaminates potable water supplies and is a suspected endocrine disrupting chemical. In our previous studies, zebrafish were exposed to 0, 0.3, 3, or 30 parts per billion (μg/L) atrazine through embryogenesis, rinsed, and allowed to mature to adulthood. A decrease in spawning was observed with morphological alterations in offspring. In addition, adult females displayed an increase in ovarian progesterone and follicular atresia, alterations in levels of a serotonin metabolite and serotonin turnover in brain tissue, and transcriptome changes in brain and ovarian tissue supporting neuroendocrine alterations. As reproductive dysfunction is also influenced by males, this study assessed testes histology, hormone levels, and transcriptomic profiles of testes and brain tissue in the adult males. The embryonic atrazine exposure resulted in no alterations in body or testes weight, gonadosomatic index, testes histology, or levels of 11-ketotestosterone or testosterone. To further investigate potential alterations, transcriptomic profiles of adult male testes and brain tissue was completed. This analysis demonstrated alterations in genes associated with abnormal cell and neuronal growth and morphology; molecular transport, quantity, and production of steroid hormones; and neurotransmission with an emphasis on the hypothalamus-pituitary-adrenal and hypothalamus-pituitary-thyroid axes. Overall, this data indicates future studies should focus on additional neuroendocrine endpoints to determine potential functional impairments.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes